| RRC ID |
51277
|
| 著者 |
Norris ML, Pauli A, Gagnon JA, Lord ND, Rogers KW, Mosimann C, Zon LI, Schier AF.
|
| タイトル |
Toddler signaling regulates mesodermal cell migration downstream of Nodal signaling.
|
| ジャーナル |
Elife
|
| Abstract |
Toddler/Apela/Elabela is a conserved secreted peptide that regulates mesendoderm development during zebrafish gastrulation. Two non-exclusive models have been proposed to explain Toddler function. The 'specification model' postulates that Toddler signaling enhances Nodal signaling to properly specify endoderm, whereas the 'migration model' posits that Toddler signaling regulates mesendodermal cell migration downstream of Nodal signaling. Here, we test key predictions of both models. We find that in toddler mutants Nodal signaling is initially normal and increasing endoderm specification does not rescue mesendodermal cell migration. Mesodermal cell migration defects in toddler mutants result from a decrease in animal pole-directed migration and are independent of endoderm. Conversely, endodermal cell migration defects are dependent on a Cxcr4a-regulated tether of the endoderm to mesoderm. These results suggest that Toddler signaling regulates mesodermal cell migration downstream of Nodal signaling and indirectly affects endodermal cell migration via Cxcr4a-signaling.
|
| 巻・号 |
6
|
| 公開日 |
2017-11-9
|
| DOI |
10.7554/eLife.22626
|
| PII |
e22626
|
| PMID |
29117894
|
| PMC |
PMC5679751
|
| MeSH |
Animals
Cell Movement*
Gene Knockout Techniques
Mesoderm / embryology*
Nodal Signaling Ligands / metabolism*
Receptors, CXCR4 / metabolism*
Signal Transduction*
Zebrafish
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism*
|
| IF |
7.08
|
| 引用数 |
8
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(sox17:EGFP) |
