論文 - 詳細
| RRC ID | 51284 |
|---|---|
| 著者 | Sato Y, Yano H, Shimizu Y, Tanaka H, Ohshima T. |
| タイトル | Optic nerve input-dependent regulation of neural stem cell proliferation in the optic tectum of adult zebrafish. |
| ジャーナル | Dev Neurobiol |
| Abstract |
Adult neurogenesis attracts broad attention as a possible cure for neurological disorders. However, its regulatory mechanism is still unclear. Therefore, they have been studying the cell proliferation mechanisms of neural stem cells (NSCs) using zebrafish, which have high regenerative potential in the adult brain. The presence of neuroepithelial-type NSCs in the optic tectum of adult zebrafish has been previously reported. In the present study, it was first confirmed that NSCs in the optic tectum decrease or increase in proportion to projection of the optic nerves from the retina. At 4 days after optic nerve crush (ONC), BrdU-positive cells decreased in the optic tectum's operation side. In contrast, at 3 weeks after ONC, BrdU-positive cells increased in the optic tectum's operation side. To study the regulatory mechanisms, they focused on the BDNF/TrkB system as a regulatory factor in the ONC model. It was found that bdnf was mainly expressed in the periventricular gray zone (PGZ) of the optic tectum by using in situ hybridization. Interestingly, expression level of bdnf significantly decreased in the optic tectum at 4 days after ONC, and its expression level tended to increase at 3 weeks after ONC. They conducted rescue experiments using a TrkB agonist and confirmed that decrease of NSC proliferation in the optic tectum by ONC was rescued by TrkB signal activation, suggesting stimuli-dependent regulation of NSC proliferation in the optic tectum of adult zebrafish. © 2016 Wiley Periodicals, Inc. Develop Neurobiol 77: 419-437, 2017. |
| 巻・号 | 77(4) |
| ページ | 474-482 |
| 公開日 | 2017-4-1 |
| DOI | 10.1002/dneu.22423 |
| PMID | 27480480 |
| MeSH | Animals Brain-Derived Neurotrophic Factor / metabolism* Cell Proliferation / physiology* Disease Models, Animal Neural Stem Cells / cytology Neural Stem Cells / physiology* Neurogenesis / physiology* Optic Nerve Injuries* Receptor, trkB / agonists Receptor, trkB / metabolism* Superior Colliculi / cytology Superior Colliculi / physiology* Zebrafish Zebrafish Proteins / metabolism* |
| IF | 3.935 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(nAChRb3:EGFP) RIKEN WT |