RRC ID 51307
Author Hill JL Jr, Josephs C, Barnes WJ, Anderson CT, Tien M.
Title Longevity in vivo of primary cell wall cellulose synthases.
Journal Plant Mol. Biol.
Abstract KEY MESSAGE:Our work focuses on understanding the lifetime and thus stability of the three main cellulose synthase (CESA) proteins involved in primary cell wall synthesis of Arabidopsis. It had long been thought that a major means of CESA regulation was via their rapid degradation. However, our studies here have uncovered that AtCESA proteins are not rapidly degraded. Rather, they persist for an extended time in the plant cell. Plant cellulose is synthesized by membrane-embedded cellulose synthase complexes (CSCs). The CSC is composed of cellulose synthases (CESAs), of which three distinct isozymes form the primary cell wall CSC and another set of three isozymes form the secondary cell wall CSC. We determined the stability over time of primary cell wall (PCW) CESAs in Arabidopsis thaliana seedlings, using immunoblotting after inhibiting protein synthesis with cycloheximide treatment. Our work reveals very slow turnover for the Arabidopsis PCW CESAs in vivo. Additionally, we show that the stability of all three CESAs within the PCW CSC is altered by mutations in individual CESAs, elevated temperature, and light conditions. Together, these results suggest that CESA proteins are very stable in vivo, but that their lifetimes can be modulated by intrinsic and environmental cues.
Volume 96(3)
Pages 279-289
Published 2018-2
DOI 10.1007/s11103-017-0695-4
PII 10.1007/s11103-017-0695-4
PMID 29388029
MeSH Arabidopsis / cytology Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cell Wall / enzymology* Cellulose / biosynthesis* Gene-Environment Interaction Glucosyltransferases / genetics Glucosyltransferases / metabolism* Mutation Seedlings / enzymology* Seedlings / genetics Seedlings / growth & development
IF 3.928
Arabidopsis / Cultured plant cells, genes pda17001