論文 - 詳細
| RRC ID | 51324 |
|---|---|
| 著者 | Cho T, Ishii-Kato A, Fukata Y, Nakayama Y, Iida K, Fukata M, Iida H. |
| タイトル | Coupling of a voltage-gated Ca2+ channel homologue with a plasma membrane H+ -ATPase in yeast. |
| ジャーナル | Genes Cells |
| Abstract |
Yeast has a homologue of mammalian voltage-gated Ca2+ channels (VGCCs), enabling the efficient uptake of Ca2+ . It comprises two indispensable subunits, Cch1 and Mid1, equivalent to the mammalian pore-forming α1 and auxiliary α2 /δ subunits, respectively. Unlike the physiological roles of Cch1/Mid1 channels, the regulatory mechanisms of the yeast VGCC homologue remain unclear. Therefore, we screened candidate proteins that interact with Mid1 by an unbiased proteomic approach and identified a plasma membrane H+ -ATPase, Pma1, as a candidate. Mid1 coimmunoprecipitated with Pma1, and Mid1-EGFP colocalized with Pma1-mCherry at the plasma membrane. The physiological relevance of their interaction was determined using the temperature-sensitive mutant, pma1-10. At the nonpermissive temperature, the membrane potential was less negative and Ca2+ uptake was lower in pma1-10 than in wild-type cells. Increased extracellular H+ increased the rate of Ca2+ uptake. Therefore, H+ extrusion by Pma1 may be important for Ca2+ influx through Cch1/Mid1. These results suggest that Pma1 interacts physically with Cch1/Mid1 Ca2+ channels to enhance their activity via its H+ -pumping activity. |
| 巻・号 | 22(1) |
| ページ | 94-104 |
| 公開日 | 2017-1-1 |
| DOI | 10.1111/gtc.12458 |
| PMID | 27935186 |
| MeSH | Amino Acid Sequence Calcium / metabolism Calcium Channels / genetics Calcium Channels / metabolism* Cell Membrane / genetics Cell Membrane / metabolism Membrane Glycoproteins / genetics Membrane Glycoproteins / metabolism* Protein Interaction Mapping / methods Proteomics* Proton-Translocating ATPases / genetics Proton-Translocating ATPases / metabolism* Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* |
| IF | 1.655 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | pBCT-CCH1H-EGFP (RDB15298) YCpT-MID1-HAx4 (RDB15299) YCpL-MID1-HAx4 (RDB15300) YCpT-MID1-EGFP (RDB15301) YCpT-PMA1-mCherry (RDB15302) YCpT-PMA1 (RDB15303). |