論文 - 詳細
| RRC ID | 51367 |
|---|---|
| 著者 | Ding WY, Ong HT, Hara Y, Wongsantichon J, Toyama Y, Robinson RC, Nédélec F, Zaidel-Bar R. |
| タイトル | Plastin increases cortical connectivity to facilitate robust polarization and timely cytokinesis. |
| ジャーナル | J Cell Biol |
| Abstract |
The cell cortex is essential to maintain animal cell shape, and contractile forces generated within it by nonmuscle myosin II (NMY-2) drive cellular morphogenetic processes such as cytokinesis. The role of actin cross-linking proteins in cortical dynamics is still incompletely understood. Here, we show that the evolutionarily conserved actin bundling/cross-linking protein plastin is instrumental for the generation of potent cortical actomyosin contractility in the Caenorhabditis elegans zygote. PLST-1 was enriched in contractile structures and was required for effective coalescence of NMY-2 filaments into large contractile foci and for long-range coordinated contractility in the cortex. In the absence of PLST-1, polarization was compromised, cytokinesis was delayed or failed, and 50% of embryos died during development. Moreover, mathematical modeling showed that an optimal amount of bundling agents enhanced the ability of a network to contract. We propose that by increasing the connectivity of the F-actin meshwork, plastin enables the cortex to generate stronger and more coordinated forces to accomplish cellular morphogenesis. |
| 巻・号 | 216(5) |
| ページ | 1371-1386 |
| 公開日 | 2017-5-1 |
| DOI | 10.1083/jcb.201603070 |
| PII | jcb.201603070 |
| PMID | 28400443 |
| PMC | PMC5412556 |
| MeSH | Actins / metabolism* Actomyosin / metabolism Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / embryology Cell Polarity* Cell Shape Cytokinesis* Membrane Glycoproteins / metabolism* Microfilament Proteins / metabolism* Morphogenesis Time Factors Zygote / cytology |
| IF | 8.811 |
| 引用数 | 30 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 32 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm4255 |