| RRC ID |
51367
|
| 著者 |
Ding WY, Ong HT, Hara Y, Wongsantichon J, Toyama Y, Robinson RC, Nédélec F, Zaidel-Bar R.
|
| タイトル |
Plastin increases cortical connectivity to facilitate robust polarization and timely cytokinesis.
|
| ジャーナル |
J Cell Biol
|
| Abstract |
The cell cortex is essential to maintain animal cell shape, and contractile forces generated within it by nonmuscle myosin II (NMY-2) drive cellular morphogenetic processes such as cytokinesis. The role of actin cross-linking proteins in cortical dynamics is still incompletely understood. Here, we show that the evolutionarily conserved actin bundling/cross-linking protein plastin is instrumental for the generation of potent cortical actomyosin contractility in the Caenorhabditis elegans zygote. PLST-1 was enriched in contractile structures and was required for effective coalescence of NMY-2 filaments into large contractile foci and for long-range coordinated contractility in the cortex. In the absence of PLST-1, polarization was compromised, cytokinesis was delayed or failed, and 50% of embryos died during development. Moreover, mathematical modeling showed that an optimal amount of bundling agents enhanced the ability of a network to contract. We propose that by increasing the connectivity of the F-actin meshwork, plastin enables the cortex to generate stronger and more coordinated forces to accomplish cellular morphogenesis.
|
| 巻・号 |
216(5)
|
| ページ |
1371-1386
|
| 公開日 |
2017-5-1
|
| DOI |
10.1083/jcb.201603070
|
| PII |
jcb.201603070
|
| PMID |
28400443
|
| PMC |
PMC5412556
|
| MeSH |
Actins / metabolism*
Actomyosin / metabolism
Animals
Caenorhabditis elegans / cytology*
Caenorhabditis elegans / embryology
Cell Polarity*
Cell Shape
Cytokinesis*
Membrane Glycoproteins / metabolism*
Microfilament Proteins / metabolism*
Morphogenesis
Time Factors
Zygote / cytology
|
| IF |
8.811
|
| 引用数 |
30
|
| リソース情報 |
| 線虫 |
tm4255 |
