論文 - 詳細
| RRC ID | 51431 |
|---|---|
| 著者 | Chang JT, Kumsta C, Hellman AB, Adams LM, Hansen M. |
| タイトル | Spatiotemporal regulation of autophagy during Caenorhabditis elegans aging. |
| ジャーナル | Elife |
| Abstract |
Autophagy has been linked to longevity in many species, but the underlying mechanisms are unclear. Using a GFP-tagged and a new tandem-tagged Atg8/LGG-1 reporter, we quantified autophagic vesicles and performed autophagic flux assays in multiple tissues of wild-type Caenorhabditis elegans and long-lived daf-2/insulin/IGF-1 and glp-1/Notch mutants throughout adulthood. Our data are consistent with an age-related decline in autophagic activity in the intestine, body-wall muscle, pharynx, and neurons of wild-type animals. In contrast, daf-2 and glp-1 mutants displayed unique age- and tissue-specific changes in autophagic activity, indicating that the two longevity paradigms have distinct effects on autophagy during aging. Although autophagy appeared active in the intestine of both long-lived mutants, inhibition of intestinal autophagy significantly abrogated lifespan extension only in glp-1 mutants. Collectively, our data suggest that autophagic activity normally decreases with age in C. elegans, whereas daf-2 and glp-1 long-lived mutants regulate autophagy in distinct spatiotemporal-specific manners to extend lifespan. |
| 巻・号 | 6 |
| 公開日 | 2017-7-4 |
| DOI | 10.7554/eLife.18459 |
| PII | e18459 |
| PMID | 28675140 |
| PMC | PMC5496740 |
| MeSH | Aging* Animal Structures / chemistry Animals Autophagosomes / metabolism Autophagy* Caenorhabditis elegans / cytology Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / analysis Microtubule-Associated Proteins / analysis Models, Animal Spatio-Temporal Analysis |
| IF | 7.08 |
| 引用数 | 53 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 42 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3489 |