RRC ID 51434
著者 Hoyt JM, Wilson SK, Kasa M, Rise JS, Topalidou I, Ailion M.
タイトル The SEK-1 p38 MAP Kinase Pathway Modulates Gq Signaling in Caenorhabditis elegans.
ジャーナル G3 (Bethesda)
Abstract Gq is a heterotrimeric G protein that is widely expressed in neurons and regulates neuronal activity. To identify pathways regulating neuronal Gq signaling, we performed a forward genetic screen in Caenorhabditis elegans for suppressors of activated Gq. One of the suppressors is an allele of sek-1, which encodes a mitogen-activated protein kinase kinase (MAPKK) in the p38 MAPK pathway. Here, we show that sek-1 mutants have a slow locomotion rate and that sek-1 acts in acetylcholine neurons to modulate both locomotion rate and Gq signaling. Furthermore, we find that sek-1 acts in mature neurons to modulate locomotion. Using genetic and behavioral approaches, we demonstrate that other components of the p38 MAPK pathway also play a positive role in modulating locomotion and Gq signaling. Finally, we find that mutants in the SEK-1 p38 MAPK pathway partially suppress an activated mutant of the sodium leak channel, NCA-1/NALCN, a downstream target of Gq signaling. Our results suggest that the SEK-1 p38 pathway may modulate the output of Gq signaling through NCA-1(unc-77).
巻・号 7(9)
ページ 2979-2989
公開日 2017-9-7
DOI 10.1534/g3.117.043273
PII g3.117.043273
PMID 28696924
PMC PMC5592925
MeSH Acetylcholine / metabolism Animals Behavior, Animal Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cholinergic Neurons / metabolism Chromosome Mapping Epistasis, Genetic GTP-Binding Protein alpha Subunits, Gq-G11 / genetics GTP-Binding Protein alpha Subunits, Gq-G11 / metabolism* Gene Expression Regulation Genome Locomotion MAP Kinase Kinase 4 / genetics MAP Kinase Kinase 4 / metabolism* Signal Transduction* Whole Genome Sequencing p38 Mitogen-Activated Protein Kinases / metabolism*
IF 2.781
引用数 2
リソース情報
線虫 tm775