論文 - 詳細
| RRC ID | 51447 |
|---|---|
| 著者 | Huang H, Zhu CT, Skuja LL, Hayden DJ, Hart AC. |
| タイトル | Genome-Wide Screen for Genes Involved in Caenorhabditis elegans Developmentally Timed Sleep. |
| ジャーナル | G3 (Bethesda) |
| Abstract |
In Caenorhabditis elegans, Notch signaling regulates developmentally timed sleep during the transition from L4 larval stage to adulthood (L4/A) . To identify core sleep pathways and to find genes acting downstream of Notch signaling, we undertook the first genome-wide, classical genetic screen focused on C. elegans developmentally timed sleep. To increase screen efficiency, we first looked for mutations that suppressed inappropriate anachronistic sleep in adult hsp::osm-11 animals overexpressing the Notch coligand OSM-11 after heat shock. We retained suppressor lines that also had defects in L4/A developmentally timed sleep, without heat shock overexpression of the Notch coligand. Sixteen suppressor lines with defects in developmentally timed sleep were identified. One line carried a new allele of goa-1; loss of GOA-1 Gαo decreased C. elegans sleep. Another line carried a new allele of gpb-2, encoding a Gβ5 protein; Gβ5 proteins have not been previously implicated in sleep. In other scenarios, Gβ5 GPB-2 acts with regulators of G protein signaling (RGS proteins) EAT-16 and EGL-10 to terminate either EGL-30 Gαq signaling or GOA-1 Gαo signaling, respectively. We found that loss of Gβ5 GPB-2 or RGS EAT-16 decreased L4/A sleep. By contrast, EGL-10 loss had no impact. Instead, loss of RGS-1 and RGS-2 increased sleep. Combined, our results suggest that, in the context of L4/A sleep, GPB-2 predominantly acts with EAT-16 RGS to inhibit EGL-30 Gαq signaling. These results confirm the importance of G protein signaling in sleep and demonstrate that these core sleep pathways function genetically downstream of the Notch signaling events promoting sleep. |
| 巻・号 | 7(9) |
| ページ | 2907-2917 |
| 公開日 | 2017-9-7 |
| DOI | 10.1534/g3.117.300071 |
| PII | g3.117.300071 |
| PMID | 28743807 |
| PMC | PMC5592919 |
| MeSH | Alleles Amino Acid Sequence Animals Animals, Genetically Modified Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics* Crosses, Genetic Gene Order Genetic Testing / methods Genome-Wide Association Study* / methods Heat-Shock Response Light Microfluidic Analytical Techniques Mutation Quantitative Trait, Heritable* Whole Genome Sequencing |
| IF | 2.781 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm761 |