RRC ID |
5155
|
著者 |
Fang F, Flynn S, Li Y, Claesson MJ, van Pijkeren JP, Collins JK, van Sinderen D, O'Toole PW.
|
タイトル |
Characterization of endogenous plasmids from Lactobacillus salivarius UCC118.
|
ジャーナル |
Appl Environ Microbiol
|
Abstract |
The genome of Lactobacillus salivarius UCC118 comprises a 1.83-Mb chromosome, a 242-kb megaplasmid (pMP118), and two smaller plasmids of 20 kb (pSF118-20) and 44 kb (pSF118-44). Annotation and bioinformatic analyses suggest that both of the smaller plasmids replicate by a theta replication mechanism. Furthermore, it appears that they are transmissible, although neither possesses a complete set of conjugation genes. Plasmid pSF118-20 encodes a toxin-antitoxin system composed of pemI and pemK homologs, and this plasmid could be cured when PemI was produced in trans. The minimal replicon of pSF118-20 was determined by deletion analysis. Shuttle vector derivatives of pSF118-20 were generated that included the replication region (pLS203) and the replication region plus mobilization genes (pLS208). The plasmid pLS203 was stably maintained without selection in Lactobacillus plantarum, Lactobacillus fermentum, and the pSF118-20-cured derivative strain of L. salivarius UCC118 (strain LS201). Cloning in pLS203 of genes encoding luciferase and green fluorescent protein, and expression from a constitutive L. salivarius promoter, demonstrated the utility of this vector for the expression of heterologous genes in Lactobacillus. This study thus expands the knowledge base and vector repertoire of probiotic lactobacilli.
|
巻・号 |
74(10)
|
ページ |
3216-28
|
公開日 |
2008-5-1
|
DOI |
10.1128/AEM.02631-07
|
PII |
AEM.02631-07
|
PMID |
18390685
|
PMC |
PMC2394924
|
MeSH |
Chromosome Mapping
Cloning, Molecular
Conjugation, Genetic
DNA Replication / genetics
Gene Deletion
Gene Order
Genes, Bacterial
Genetic Vectors
Green Fluorescent Proteins / genetics
Lactobacillus / genetics*
Luciferases / genetics
Physical Chromosome Mapping
Plasmids / isolation & purification*
Replicon
|
IF |
4.016
|
引用数 |
37
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM1040
JCM1042
JCM1045
JCM1046
JCM1230
JCM12378
JCM12379 |