RRC ID 5155
著者 Fang F, Flynn S, Li Y, Claesson MJ, van Pijkeren JP, Collins JK, van Sinderen D, O'Toole PW.
タイトル Characterization of endogenous plasmids from Lactobacillus salivarius UCC118.
ジャーナル Appl Environ Microbiol
Abstract The genome of Lactobacillus salivarius UCC118 comprises a 1.83-Mb chromosome, a 242-kb megaplasmid (pMP118), and two smaller plasmids of 20 kb (pSF118-20) and 44 kb (pSF118-44). Annotation and bioinformatic analyses suggest that both of the smaller plasmids replicate by a theta replication mechanism. Furthermore, it appears that they are transmissible, although neither possesses a complete set of conjugation genes. Plasmid pSF118-20 encodes a toxin-antitoxin system composed of pemI and pemK homologs, and this plasmid could be cured when PemI was produced in trans. The minimal replicon of pSF118-20 was determined by deletion analysis. Shuttle vector derivatives of pSF118-20 were generated that included the replication region (pLS203) and the replication region plus mobilization genes (pLS208). The plasmid pLS203 was stably maintained without selection in Lactobacillus plantarum, Lactobacillus fermentum, and the pSF118-20-cured derivative strain of L. salivarius UCC118 (strain LS201). Cloning in pLS203 of genes encoding luciferase and green fluorescent protein, and expression from a constitutive L. salivarius promoter, demonstrated the utility of this vector for the expression of heterologous genes in Lactobacillus. This study thus expands the knowledge base and vector repertoire of probiotic lactobacilli.
巻・号 74(10)
ページ 3216-28
公開日 2008-5-1
DOI 10.1128/AEM.02631-07
PII AEM.02631-07
PMID 18390685
PMC PMC2394924
MeSH Chromosome Mapping Cloning, Molecular Conjugation, Genetic DNA Replication / genetics Gene Deletion Gene Order Genes, Bacterial Genetic Vectors Green Fluorescent Proteins / genetics Lactobacillus / genetics* Luciferases / genetics Physical Chromosome Mapping Plasmids / isolation & purification* Replicon
IF 4.016
引用数 37
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM1040 JCM1042 JCM1045 JCM1046 JCM1230 JCM12378 JCM12379