| RRC ID |
51556
|
| 著者 |
Kim KW, Tang NH, Andrusiak MG, Wu Z, Chisholm AD, Jin Y.
|
| タイトル |
A Neuronal piRNA Pathway Inhibits Axon Regeneration in C. elegans.
|
| ジャーナル |
Neuron
|
| Abstract |
The PIWI-interacting RNA (piRNA) pathway has long been thought to function solely in the germline, but evidence for its functions in somatic cells is emerging. Here we report an unexpected role for the piRNA pathway in Caenorhabditis elegans sensory axon regeneration after injury. Loss of function in a subset of components of the piRNA pathway results in enhanced axon regrowth. Two essential piRNA factors, PRDE-1 and PRG-1/PIWI, inhibit axon regeneration in a gonad-independent and cell-autonomous manner. By smFISH analysis we find that prde-1 transcripts are present in neurons, as well as germ cells. The piRNA pathway inhibits axon regrowth independent of nuclear transcriptional silencing but dependent on the slicer domain of PRG-1/PIWI, suggesting that post-transcriptional gene silencing is involved. Our results reveal the neuronal piRNA pathway as a novel intrinsic repressor of axon regeneration.
|
| 巻・号 |
97(3)
|
| ページ |
511-519.e6
|
| 公開日 |
2018-2-7
|
| DOI |
10.1016/j.neuron.2018.01.014
|
| PII |
S0896-6273(18)30017-5
|
| PMID |
29395906
|
| PMC |
PMC5866297
|
| MeSH |
Animals
Argonaute Proteins / metabolism*
Axons / metabolism*
Caenorhabditis elegans
Caenorhabditis elegans Proteins / metabolism*
Germ Cells / metabolism
RNA, Small Interfering / metabolism*
Regeneration*
Signal Transduction
|
| IF |
14.415
|
| 引用数 |
18
|
| リソース情報 |
| 線虫 |
tm1116
tm1200
tm1489
tm2252
tm3748
tm4477
tm6145
tm6424
tm869
tm872 |
