RRC ID 51556
Author Kim KW, Tang NH, Andrusiak MG, Wu Z, Chisholm AD, Jin Y.
Title A Neuronal piRNA Pathway Inhibits Axon Regeneration in C. elegans.
Journal Neuron
Abstract The PIWI-interacting RNA (piRNA) pathway has long been thought to function solely in the germline, but evidence for its functions in somatic cells is emerging. Here we report an unexpected role for the piRNA pathway in Caenorhabditis elegans sensory axon regeneration after injury. Loss of function in a subset of components of the piRNA pathway results in enhanced axon regrowth. Two essential piRNA factors, PRDE-1 and PRG-1/PIWI, inhibit axon regeneration in a gonad-independent and cell-autonomous manner. By smFISH analysis we find that prde-1 transcripts are present in neurons, as well as germ cells. The piRNA pathway inhibits axon regrowth independent of nuclear transcriptional silencing but dependent on the slicer domain of PRG-1/PIWI, suggesting that post-transcriptional gene silencing is involved. Our results reveal the neuronal piRNA pathway as a novel intrinsic repressor of axon regeneration.
Volume 97(3)
Pages 511-519.e6
Published 2018-2-7
DOI 10.1016/j.neuron.2018.01.014
PII S0896-6273(18)30017-5
PMID 29395906
PMC PMC5866297
MeSH Animals Argonaute Proteins / metabolism* Axons / metabolism* Caenorhabditis elegans Caenorhabditis elegans Proteins / metabolism* Germ Cells / metabolism RNA, Small Interfering / metabolism* Regeneration* Signal Transduction
IF 14.415
Times Cited 18
C.elegans tm1116 tm1200 tm1489 tm2252 tm3748 tm4477 tm6145 tm6424 tm869 tm872