RRC ID 51586
Author Samatiwat P, Takeda K, Satarug S, Ohba K, Kukongviriyapan V, Shibahara S.
Title Induction of MITF expression in human cholangiocarcinoma cells and hepatocellular carcinoma cells by cyclopamine, an inhibitor of the Hedgehog signaling.
Journal Biochem. Biophys. Res. Commun.
Abstract Microphthalmia-associated transcription factor (MITF) is a key regulator of differentiation of melanocytes and retinal pigment epithelial cells, but it also has functions in non-pigment cells. MITF consists of multiple isoforms, including widely expressed MITF-A and MITF-H. In the present study, we explored the potential role played by the Hedgehog signaling on MITF expression in two common types of primary liver cancer, using human cholangiocarcinoma cell lines, the KKU-100 and HuCCT1, along with the HepG2 human hepatocellular carcinoma cell line. Importantly, cholangiocarcinoma is characterized by the activated Hedgehog signaling. Here we show that MITF-A mRNA is predominantly expressed in all three human liver cancer cell lines examined. Moreover, cyclopamine, an inhibitor of the Hedgehog signalling, increased the expression levels of MITF proteins in HuCCT1 and HepG2 cells, but not in KKU-100 cells, suggesting that MITF expression may be down-regulated in some liver cancer cases.
Volume 470(1)
Pages 144-149
Published 2016-1-29
DOI 10.1016/j.bbrc.2016.01.010
PII S0006-291X(16)30010-9
PMID 26773496
MeSH Carcinoma, Hepatocellular / metabolism* Cell Line, Tumor Cell Survival / drug effects Cholangiocarcinoma / metabolism* Down-Regulation / drug effects Hedgehog Proteins / metabolism* Hep G2 Cells Humans Liver Neoplasms / metabolism* Microphthalmia-Associated Transcription Factor / metabolism* Signal Transduction / drug effects Veratrum Alkaloids / administration & dosage*
IF 2.705
Human and Animal Cells HuCCT1(RCB1960)