RRC ID 51615
Author Yasui H, Imura Y, Outani H, Hamada K, Nakai T, Yamada S, Takenaka S, Sasagawa S, Araki N, Itoh K, Myoui A, Yoshikawa H, Naka N.
Title Trabectedin is a promising antitumour agent for synovial sarcoma.
Journal J Chemother
Abstract Synovial sarcoma (SS) is an aggressive soft tissue tumour with poor prognosis. Using five human SS cell lines, we examined the cytotoxic effects of trabectedin (ET-743; Yondelis(®)), a novel marine natural product, which was approved in Europe for the treatment of soft tissue sarcomas (STS). The significant growth inhibitory effects were observed in all SS cell lines below nanomolar concentration of trabectedin. Furthermore, trabectedin significantly suppressed the tumour growth in xenograft models. Flow cytometer analysis in vitro and immunohistochemical analysis in vivo revealed its effect of cell cycle inhibition and apoptosis induction. We also examined the expression of ERCC1, 5 and BRCA1 in SS cell lines and clinical samples, and majority of them showed highly trabectedin-sensitive pattern as previously reported in other cancers. Our preclinical data indicated that trabectedin could be a promising therapeutic option for patients with SS.
Volume 28(5)
Pages 417-24
Published 2016-10
DOI 10.1080/1120009X.2015.1133013
PMID 27077926
MeSH Adolescent Adult Animals Antineoplastic Agents / pharmacology* Apoptosis / drug effects BRCA1 Protein / analysis BRCA1 Protein / biosynthesis Biomarkers, Tumor / analysis Blotting, Western Cell Cycle / drug effects Cell Line, Tumor Cell Proliferation / drug effects DNA-Binding Proteins / analysis DNA-Binding Proteins / biosynthesis Dioxoles / pharmacology* Endonucleases / analysis Endonucleases / biosynthesis Female Flow Cytometry Humans Immunohistochemistry Male Mice Middle Aged Nuclear Proteins / analysis Nuclear Proteins / biosynthesis Sarcoma, Synovial / pathology* Tetrahydroisoquinolines / pharmacology* Trabectedin Transcription Factors / analysis Transcription Factors / biosynthesis Xenograft Model Antitumor Assays Young Adult
IF 1.49
Human and Animal Cells HS-SY-II(RCB2231)