RRC ID 51638
Author Fujiwara Y, Yang L, Takaiwa F, Sekikawa K.
Title Expression and Purification of Recombinant Mouse Interleukin-4 and -6 from Transgenic Rice Seeds.
Journal Mol Biotechnol
Abstract Transgenic rice seed can be utilized as a bioreactor to produce high-value recombinant proteins. Mouse interleukin 4 (mIL-4) and mIL-6 were specifically expressed as secretory proteins in rice endosperm by ligating the N-terminal glutelin B-1 (GluB-1) signal peptide and the C-terminal KDEL endoplasmic reticulum retention signal under control of the endosperm-specific GluB-1 promoter. In the transgenic rice seed, mIL-4 and mIL-6 accumulated in levels up to 0.43 mg/g grain and 0.16 mg/g grain, respectively. The reducing agents and detergents required for extraction from the transgenic rice seeds differed between the two proteins, indicating differences in their intracellular localization within the endosperm cell. Purified mIL-4 and mIL-6 exhibited high activity and very low endotoxin contamination.
Volume 58(4)
Pages 223-31
Published 2016-4-1
DOI 10.1007/s12033-016-9920-7
PII 10.1007/s12033-016-9920-7
PMID 26876890
MeSH Animals Detergents Interleukin-4 / genetics* Interleukin-4 / isolation & purification* Interleukin-4 / metabolism Interleukin-6 / genetics* Interleukin-6 / isolation & purification* Interleukin-6 / metabolism Mice Oryza / genetics* Oryza / growth & development Plants, Genetically Modified / growth & development Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism Seeds / genetics* Seeds / metabolism
IF 2.022
Times Cited 5
Human and Animal Cells 7-TD-1(RCB1190)