論文 - 詳細
RRC ID | 51662 |
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著者 | Chang HS, Tang JY, Yen CY, Huang HW, Wu CY, Chung YA, Wang HR, Chen IS, Huang MY, Chang HW. |
タイトル | Antiproliferation of Cryptocarya concinna-derived cryptocaryone against oral cancer cells involving apoptosis, oxidative stress, and DNA damage. |
ジャーナル | BMC Complement Altern Med |
Abstract |
BACKGROUND:Cryptocarya-derived crude extracts and their compounds have been reported to have an antiproliferation effect on several types of cancers but their impact on oral cancer is less well understood. METHODS:We examined the cell proliferation effect and mechanism of C. concinna-derived cryptocaryone (CPC) on oral cancer cells in terms of cell viability, apoptosis, reactive oxygen species (ROS), mitochondrial depolarization, and DNA damage. RESULTS:We found that CPC dose-responsively reduced cell viability of two types of oral cancer cells (Ca9-22 and CAL 27) in MTS assay. The CPC-induced dose-responsive apoptosis effects on Ca9-22 cells were confirmed by flow cytometry-based sub-G1 accumulation, annexin V staining, and pancaspase analyses. For oral cancer Ca9-22 cells, CPC also induced oxidative stress responses in terms of ROS generation and mitochondrial depolarization. Moreover, γH2AX flow cytometry showed DNA damage in CPC-treated Ca9-22 cells. CPC-induced cell responses in terms of cell viability, apoptosis, oxidative stress, and DNA damage were rescued by N-acetylcysteine pretreatment, suggesting that oxidative stress plays an important role in CPC-induced death of oral cancer cells. CONCLUSIONS:CPC is a potential ROS-mediated natural product for anti-oral cancer therapy. |
巻・号 | 16 |
ページ | 94 |
公開日 | 2016-3-8 |
DOI | 10.1186/s12906-016-1073-5 |
PII | 10.1186/s12906-016-1073-5 |
PMID | 26955958 |
PMC | PMC4784356 |
MeSH | Antineoplastic Agents, Phytogenic / pharmacology Antineoplastic Agents, Phytogenic / therapeutic use* Apoptosis Cell Line, Tumor Cell Proliferation Cryptocarya / chemistry* DNA Damage Humans Mouth Neoplasms / drug therapy* Oxidative Stress Phytotherapy* Plant Extracts / pharmacology Plant Extracts / therapeutic use* Pyrones / pharmacology Pyrones / therapeutic use* |
IF | 2.833 |
引用数 | 9 |
リソース情報 | |
ヒト・動物細胞 | Ca9-22(RCB1976) |