論文 - 詳細
| RRC ID | 51708 |
|---|---|
| 著者 | Tarhan YE, Kato T, Jang M, Haga Y, Ueda K, Nakamura Y, Park JH. |
| タイトル | Morphological Changes, Cadherin Switching, and Growth Suppression in Pancreatic Cancer by GALNT6 Knockdown. |
| ジャーナル | Neoplasia |
| Abstract |
Pancreatic cancer reveals the worst prognosis among human cancers with little improvement in its clinical outcome in the last three decades. We previously suggested that polypeptide N-acetylgalactosaminyltransferase 6 (GALNT6), which catalyzes O-type glycosylation of Mucin 1, might be a promising molecular target for drug development for breast cancer. In this study, we report upregulation of GALNT6 in pancreatic cancer cells where Mucin proteins are highly O-glycosylated. We found that knockdown of GALNT6 with small interfering RNA in pancreatic cancer cells decreased the amount of Mucin 4 protein as well as that of its transcript, reduced the levels of human epidermal growth factor receptor 2 and extracellular signal-regulated kinase, and significantly reduced pancreatic cancer cell viability. Interestingly, knockdown of GALNT6 caused drastic morphological changes of pancreatic cells, accompanied with the cadherin switching from P-cadherin to E-cadherin. Considering important roles of Mucin 4 in growth and invasion, our findings imply that targeting GALNT6 is a very promising therapeutic strategy for treatment of pancreatic cancer patients who still have very limited treatment modalities. |
| 巻・号 | 18(5) |
| ページ | 265-272 |
| 公開日 | 2016-5-1 |
| DOI | 10.1016/j.neo.2016.03.005 |
| PII | S1476-5586(16)30003-3 |
| PMID | 27237318 |
| PMC | PMC4887616 |
| MeSH | Cadherins / metabolism* Cell Line, Tumor Cell Survival / genetics Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Gene Silencing Glycosylation Humans Mucin-4 / genetics Mucin-4 / metabolism N-Acetylgalactosaminyltransferases / deficiency* N-Acetylgalactosaminyltransferases / genetics Pancreatic Neoplasms / genetics* Pancreatic Neoplasms / metabolism* Pancreatic Neoplasms / pathology RNA, Small Interfering / genetics Signal Transduction |
| IF | 5.696 |
| 引用数 | 20 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | KP4(RCB1005) |