論文 - 詳細
| RRC ID | 5172 |
|---|---|
| 著者 | Okuda K, Aso Y, Nakayama J, Sonomoto K. |
| タイトル | Cooperative transport between NukFEG and NukH in immunity against the lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1. |
| ジャーナル | J Bacteriol |
| Abstract |
Nukacin ISK-1 is a lantibiotic produced by Staphylococcus warneri ISK-1. Previous studies have reported that the self-protection system of the nukacin ISK-1 producer involves the cooperative function of the ABC transporter NukFEG and the lantibiotic-binding immunity protein NukH. In this study, the cooperative mechanism between NukFEG and NukH was characterized by using fluorescein-4-isothiocyanate (FITC)-labeled nukacin ISK-1 (FITC-nuk) to clarify the localization of nukacin ISK-1 in the immunity process. Lactococcus lactis recombinants expressing nukFEGH, nukFEG, or nukH showed immunity against FITC-nuk, suggesting that FITC-nuk was recognized by the self-protection system against nukacin ISK-1. Analysis of the interaction between FITC-nuk and energy-deprived cells of the L. lactis recombinants showed that FITC-nuk specifically bound to cells expressing nukH. The interaction between FITC-nuk and nukH-expressing cells was inhibited by the addition of unlabeled nukacin ISK-1 and its derivatives with deletions of the N-terminal tail region, but not by the addition of a synthesized N-terminal tail region. This suggests that the NukH protein recognizes the C-terminal ring region of nukacin ISK-1. The addition of glucose to nukFEGH-expressing cells treated with FITC-nuk resulted in a time-dependent decrease in fluorescence intensity, indicating that FITC-nuk was transported from the cell membrane by the NukFEG protein. These results revealed that after being captured by NukH in an energy-independent manner, nukacin ISK-1 was transported to the extracellular space by NukFEG in an energy-dependent manner. |
| 巻・号 | 190(1) |
| ページ | 356-62 |
| 公開日 | 2008-1-1 |
| DOI | 10.1128/JB.01300-07 |
| PII | JB.01300-07 |
| PMID | 17951378 |
| PMC | PMC2223764 |
| MeSH | ATP-Binding Cassette Transporters / drug effects ATP-Binding Cassette Transporters / metabolism* Bacterial Proteins / drug effects Bacterial Proteins / metabolism* Bacteriocins / metabolism* Biological Transport Kinetics Lactobacillus / drug effects Lactobacillus / metabolism Leuconostoc / drug effects Leuconostoc / metabolism Microscopy, Fluorescence Staphylococcus / genetics Staphylococcus / immunology Staphylococcus / physiology* |
| IF | 3.006 |
| 引用数 | 25 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM1157 JCM5885 JCM6124 |