RRC ID 51735
著者 Sasaki S, Kanzaki M, Kaneko T.
タイトル Calcium influx through TRP channels induced by short-lived reactive species in plasma-irradiated solution.
ジャーナル Sci Rep
Abstract Non-equilibrium helium atmospheric-pressure plasma (He-APP), which allows for a strong non-equilibrium chemical reaction of O2 and N2 in ambient air, uniquely produces multiple extremely reactive products, such as reactive oxygen species (ROS), in plasma-irradiated solution. We herein show that relatively short-lived unclassified reactive species (i.e., deactivated within approximately 10 min) generated by the He-APP irradiation can trigger physiologically relevant Ca(2+) influx through ruthenium red- and SKF 96365-sensitive Ca(2+)-permeable channel(s), possibly transient receptor potential channel family member(s). Our results provide novel insight into understanding of the interactions between cells and plasmas and the mechanism by which cells detect plasma-induced chemically reactive species, in addition to facilitating development of plasma applications in medicine.
巻・号 6
ページ 25728
公開日 2016-5-12
DOI 10.1038/srep25728
PII srep25728
PMID 27169489
PMC PMC4864414
MeSH 3T3-L1 Cells Animals Atmospheric Pressure Calcium / metabolism* Glucose / pharmacology Helium / pharmacology Hydrogen Peroxide / metabolism Hydroxyl Radical / metabolism Imidazoles / metabolism Mannitol / pharmacology Mice Plasma Gases / pharmacology* Reactive Oxygen Species / metabolism* Ruthenium Red / metabolism Single-Cell Analysis Solutions Time Factors Time-Lapse Imaging Transient Receptor Potential Channels / metabolism*
IF 3.998
引用数 19
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リソース情報
ヒト・動物細胞 MCF7(RCB1904)