Reference - Detail
| RRC ID | 51740 |
|---|---|
| Author | Horiguchi I, Sakai Y. |
| Title | Serum replacement with albumin-associated lipids prevents excess aggregation and enhances growth of induced pluripotent stem cells in suspension culture. |
| Journal | Biotechnol Prog |
| Abstract |
Suspension culture systems are currently under investigation for the mass production of pluripotent stem (PS) cells for tissue engineering; however, the control of cell aggregation in suspension culture remains challenging. Existing methods to control aggregation such as microwell culture are difficult to scale up. To address this issue, in this study a novel method that incorporates the addition of KnockOut Serum Replacement (KSR) to the PS cell culture medium was described. The method regulated cellular aggregation and significantly improved cell growth (a 2- to 10-fold increase) without any influence on pluripotency. In addition, albumin-associated lipids as the major working ingredient of KSR responsible for this inhibition of aggregation were identified. This is one of the simplest methods described to date to control aggregation and requires only chemically synthesizable reagents. Thus, this method has the potential to simplify the mass production process of PS cells and thus lower their cost. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1009-1016, 2016. |
| Volume | 32(4) |
| Pages | 1009-16 |
| Published | 2016-7-8 |
| DOI | 10.1002/btpr.2301 |
| PMID | 27193385 |
| MeSH | Albumins / chemistry* Cell Aggregation / drug effects Cell Culture Techniques* Cells, Cultured Culture Media, Conditioned / chemistry Culture Media, Conditioned / pharmacology* Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / drug effects* Lipids / blood Lipids / chemistry Lipids / pharmacology* |
| IF | 2.334 |
| Times Cited | 4 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 201B7(HPS0063) |