RRC ID |
51741
|
著者 |
Kudo M, Katayoshi T, Kobayashi-Nakamura K, Akagawa M, Tsuji-Naito K.
|
タイトル |
H(+)/peptide transporter (PEPT2) is expressed in human epidermal keratinocytes and is involved in skin oligopeptide transport.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Peptide transporter 2 (PEPT2) is a member of the proton-coupled oligopeptide transporter family, which mediates the cellular uptake of oligopeptides and peptide-like drugs. Although PEPT2 is expressed in many tissues, its expression in epidermal keratinocytes remains unclear. We investigated PEPT2 expression profile and functional activity in keratinocytes. We confirmed PEPT2 mRNA expression in three keratinocyte lines (normal human epidermal keratinocytes (NHEKs), immortalized keratinocytes, and malignant keratinocytes) by reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time RT-PCR. In contrast to PEPT1, PEPT2 expression in the three keratinocytes was similar or higher than that in HepG2 cells, used as PEPT2-positive cells. Immunolocalization analysis using human skin showed epidermal PEPT2 localization. We studied keratinocyte transport function by measuring the oligopeptide content using liquid chromatography/tandem mass spectrometry. Glycylsarcosine uptake in NHEKs was pH-dependent, suggesting that keratinocytes could absorb small peptides in the presence of an inward H(+) gradient. We also performed a skin-permeability test of several oligopeptides using skin substitute, suggesting that di- and tripeptides pass actively through the epidermis. In conclusion, PEPT2 is expressed in keratinocytes and involved in skin oligopeptide uptake.
|
巻・号 |
475(4)
|
ページ |
335-41
|
公開日 |
2016-7-8
|
DOI |
10.1016/j.bbrc.2016.05.093
|
PII |
S0006-291X(16)30801-4
|
PMID |
27216463
|
MeSH |
Adult
Biological Transport
Cells, Cultured
Epidermal Cells
Epidermis / metabolism*
Female
Gene Expression
Humans
Keratinocytes / cytology
Keratinocytes / metabolism*
Male
Oligopeptides / metabolism*
RNA, Messenger / analysis
RNA, Messenger / genetics
Symporters / analysis
Symporters / genetics*
Symporters / metabolism*
|
IF |
2.985
|
引用数 |
9
|
リソース情報 |
ヒト・動物細胞 |
CACO-2(RCB0988) |