| RRC ID |
51753
|
| 著者 |
Shirouzu Y, Yanai G, Yang KC, Sumi S.
|
| タイトル |
Effects of Activin in Embryoid Bodies Expressing Fibroblast Growth Factor 5.
|
| ジャーナル |
Cell Reprogram
|
| Abstract |
Nodal/activin signaling is indispensable for embryonic development. We examined what activin does to the embryoid bodies (EBs) produced from mouse embryonic stem cells (mESCs) expressing an epiblast marker. The EBs were produced by culturing mESCs by the hanging drop method for 24 hours. The resulting EBs were transferred onto gelatin-coated dishes and allowed to further differentiate. The 24-hour EBs showed a stronger expression of fibroblast growth factor (FGF)5 and Brachyury (specific to the epiblast) in comparison with mESCs. Treating the transferred EBs with activin A maintained transcript levels of FGF5 and Oct4, while inhibiting definitive endoderm differentiation. The activin A treatment reversed the endoderm differentiation induced by retinoic acid (RA), while the inhibition of nodal/activin signaling promoted RA-induced endoderm differentiation. Inhibition of nodal/activin signaling in EBs, including epiblast-like cells, promotes differentiation into the endoderm, facilitating the transition from the pluripotent state to specification of the endoderm.
|
| 巻・号 |
18(3)
|
| ページ |
171-86
|
| 公開日 |
2016-6-1
|
| DOI |
10.1089/cell.2015.0074
|
| PMID |
27253628
|
| MeSH |
Activins / pharmacology*
Animals
Cells, Cultured
Cellular Reprogramming*
Embryoid Bodies / cytology
Embryoid Bodies / drug effects*
Endoderm / drug effects*
Fetal Proteins / genetics
Fetal Proteins / metabolism
Fibroblast Growth Factor 5 / genetics
Fibroblast Growth Factor 5 / metabolism*
Gene Expression Regulation, Developmental
Mice
Octamer Transcription Factor-3 / genetics
RNA, Messenger / genetics
RNA, Messenger / metabolism
Signal Transduction
T-Box Domain Proteins / genetics
T-Box Domain Proteins / metabolism
|
| IF |
1.702
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
iPS-MEF-Ng-20D-17(APS0001) |
