| RRC ID |
51801
|
| 著者 |
Li JR, Suzuki T, Nishimura H, Kishima M, Maeda S, Suzuki H.
|
| タイトル |
Asymmetric Regulation of Peripheral Genes by Two Transcriptional Regulatory Networks.
|
| ジャーナル |
PLoS One
|
| Abstract |
Transcriptional regulatory network (TRN) reconstitution and deconstruction occur simultaneously during reprogramming; however, it remains unclear how the starting and targeting TRNs regulate the induction and suppression of peripheral genes. Here we analyzed the regulation using direct cell reprogramming from human dermal fibroblasts to monocytes as the platform. We simultaneously deconstructed fibroblastic TRN and reconstituted monocytic TRN; monocytic and fibroblastic gene expression were analyzed in comparison with that of fibroblastic TRN deconstruction only or monocytic TRN reconstitution only. Global gene expression analysis showed cross-regulation of TRNs. Detailed analysis revealed that knocking down fibroblastic TRN positively affected half of the upregulated monocytic genes, indicating that intrinsic fibroblastic TRN interfered with the expression of induced genes. In contrast, reconstitution of monocytic TRN showed neutral effects on the majority of fibroblastic gene downregulation. This study provides an explicit example that demonstrates how two networks together regulate gene expression during cell reprogramming processes and contributes to the elaborate exploration of TRNs.
|
| 巻・号 |
11(8)
|
| ページ |
e0160459
|
| 公開日 |
2016-1-1
|
| DOI |
10.1371/journal.pone.0160459
|
| PII |
PONE-D-16-17190
|
| PMID |
27483142
|
| PMC |
PMC4970704
|
| MeSH |
Cell Line
Cellular Reprogramming*
Fibroblasts / cytology
Fibroblasts / metabolism*
Gene Expression Profiling
Gene Expression Regulation
Gene Regulatory Networks*
Genetic Vectors / chemistry
Genetic Vectors / metabolism
Homeodomain Proteins / genetics
Homeodomain Proteins / metabolism
Humans
LIM-Homeodomain Proteins / genetics
LIM-Homeodomain Proteins / metabolism
Lentivirus / genetics
Lentivirus / metabolism
Microarray Analysis
Monocytes / cytology
Monocytes / metabolism*
Repressor Proteins / genetics
Repressor Proteins / metabolism
Skin / cytology
Skin / metabolism
Transcription Factors / genetics
Transcription Factors / metabolism
Transcription, Genetic*
Transduction, Genetic
Twist-Related Protein 1 / genetics
Twist-Related Protein 1 / metabolism
|
| IF |
2.74
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
NB1RGB(RCB0222) |
