RRC ID 51809
著者 Koyama S, Narita E, Shimizu Y, Shiina T, Taki M, Shinohara N, Miyakoshi J.
タイトル Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells.
ジャーナル Int J Environ Res Public Health
Abstract To investigate the cellular effects of terahertz (THz) exposure, human corneal epithelial (HCE-T) cells derived from human eye were exposed to 0.12 THz radiation at 5 mW/cm² for 24 h, then the genotoxicity, morphological changes, and heat shock protein (Hsp) expression of the cells were examined. There was no statistically significant increase in the micronucleus (MN) frequency of cells exposed to 0.12 THz radiation compared with sham-exposed controls and incubator controls, whereas the MN frequency of cells treated with bleomycin for 1 h (positive control) did increase significantly. Similarly, there were no significant morphological changes in cells exposed to 0.12 THz radiation compared to sham-exposed controls and incubator controls, and Hsp expression (Hsp27, Hsp70, and Hsp90α) was also not significantly different between the three treatments. These results indicate that exposure to 0.12 THz radiation using the present conditions appears to have no or very little effect on MN formation, morphological changes, and Hsp expression in cells derived from human eye.
巻・号 13(8)
公開日 2016-8-5
DOI 10.3390/ijerph13080793
PII ijerph13080793
PMID 27527204
PMC PMC4997479
MeSH Blotting, Western Cell Line DNA Damage* Electromagnetic Fields / adverse effects* Epithelial Cells / cytology* Epithelium, Corneal / cytology* HSP27 Heat-Shock Proteins / genetics HSP27 Heat-Shock Proteins / metabolism HSP70 Heat-Shock Proteins / genetics HSP70 Heat-Shock Proteins / metabolism HSP90 Heat-Shock Proteins / genetics HSP90 Heat-Shock Proteins / metabolism Heat-Shock Proteins / genetics* Heat-Shock Proteins / metabolism Humans Micronucleus Tests Molecular Chaperones Time Factors
IF 2.849
引用数 7
リソース情報
ヒト・動物細胞 HCE-T(RCB2280)