RRC ID 51814
Author Kusuyama J, Komorizono A, Bandow K, Ohnishi T, Matsuguchi T.
Title CXCL3 positively regulates adipogenic differentiation.
Journal J Lipid Res
Abstract Chemokines are a family of cytokines inducing cell migration and inflammation. Recent reports have implicated the roles of chemokines in cell differentiation. However, little is known about the functional roles of chemokines in adipocytes. Here, we explored gene expression levels of chemokines and chemokine receptors during adipogenic differentiation. We have found that two chemokines, chemokine (C-X-C motif) ligand 3 (CXCL3) and CXCL13, as well as CXC chemokine receptor 2 (CXCR2), a CXCL3 receptor, are highly expressed in mature adipocytes. When 3T3-L1 cells and ST2 cells were induced to differentiate, both the number of lipid droplets and the expression levels of adipogenic markers were significantly promoted by the addition of CXCL3, but not CXCL13. Conversely, gene knockdown of either CXCL3 or CXCR2 by specific siRNA effectively inhibited the course of adipogenic differentiation. CXCL3 treatment of 3T3-L1 cells significantly induced the phosphorylation of ERK and c-jun N-terminal kinase (JNK). Furthermore, CXCL3-induced CCAAT-enhancer binding protein (C/EBP)β and δ expression was suppressed by both ERK and JNK-specific inhibitors. Furthermore, chromatin immunoprecipitation assay revealed functional binding of PPARγ2 within the cxcl3 promoter region. Taken together, these results have indicated that CXCL3 is a novel adipokine that facilitates adipogenesis in an autocrine and/or a paracrine manner through induction of c/ebpb and c/ebpd.
Volume 57(10)
Pages 1806-1820
Published 2016-10-1
DOI 10.1194/jlr.M067207
PII jlr.M067207
PMID 27512010
PMC PMC5036360
MeSH 3T3-L1 Cells Adipogenesis / physiology* Adipokines / biosynthesis* Adipokines / genetics Animals CCAAT-Enhancer-Binding Protein-beta / genetics CCAAT-Enhancer-Binding Protein-beta / metabolism CCAAT-Enhancer-Binding Protein-delta / genetics CCAAT-Enhancer-Binding Protein-delta / metabolism Cell Differentiation / physiology* Chemokine CXCL13 / genetics Chemokine CXCL13 / metabolism Chemokines, CXC / biosynthesis* Chemokines, CXC / genetics Extracellular Signal-Regulated MAP Kinases / genetics Extracellular Signal-Regulated MAP Kinases / metabolism JNK Mitogen-Activated Protein Kinases / genetics JNK Mitogen-Activated Protein Kinases / metabolism MAP Kinase Signaling System / physiology* Mice PPAR gamma / genetics PPAR gamma / metabolism Paracrine Communication / physiology* Promoter Regions, Genetic Receptors, Interleukin-8B / genetics Receptors, Interleukin-8B / metabolism
IF 4.483
Times Cited 16
Human and Animal Cells ST2(RCB0224)