| RRC ID |
51876
|
| 著者 |
Mora-Bermúdez F, Badsha F, Kanton S, Camp JG, Vernot B, Köhler K, Voigt B, Okita K, Maricic T, He Z, Lachmann R, Pääbo S, Treutlein B, Huttner WB.
|
| タイトル |
Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development.
|
| ジャーナル |
Elife
|
| Abstract |
Human neocortex expansion likely contributed to the remarkable cognitive abilities of humans. This expansion is thought to primarily reflect differences in proliferation versus differentiation of neural progenitors during cortical development. Here, we have searched for such differences by analysing cerebral organoids from human and chimpanzees using immunohistofluorescence, live imaging, and single-cell transcriptomics. We find that the cytoarchitecture, cell type composition, and neurogenic gene expression programs of humans and chimpanzees are remarkably similar. Notably, however, live imaging of apical progenitor mitosis uncovered a lengthening of prometaphase-metaphase in humans compared to chimpanzees that is specific to proliferating progenitors and not observed in non-neural cells. Consistent with this, the small set of genes more highly expressed in human apical progenitors points to increased proliferative capacity, and the proportion of neurogenic basal progenitors is lower in humans. These subtle differences in cortical progenitors between humans and chimpanzees may have consequences for human neocortex evolution.
|
| 巻・号 |
5
|
| 公開日 |
2016-9-26
|
| DOI |
10.7554/eLife.18683
|
| PII |
e18683
|
| PMID |
27669147
|
| PMC |
PMC5110243
|
| MeSH |
Animals
Cell Proliferation
Cerebral Cortex / embryology*
Gene Expression Profiling
Humans
Intravital Microscopy
Microscopy, Fluorescence
Mitosis
Neural Stem Cells / physiology*
Organoids / growth & development
Pan troglodytes
Single-Cell Analysis
|
| IF |
7.08
|
| 引用数 |
66
|
| リソース情報 |
| ヒト・動物細胞 |
409B2(HPS0076) |
