| RRC ID |
5192
|
| 著者 |
Soto-Ramírez N, Sánchez-Porro C, Rosas-Padilla S, Almodóvar K, Jiménez G, Machado-Rodríguez M, Zapata M, Ventosa A, Montalvo-Rodríguez R.
|
| タイトル |
Halobacillus mangrovi sp. nov., a moderately halophilic bacterium isolated from the black mangrove Avicennia germinans.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A moderately halophilic, spore-forming, Gram-positive, short-rod-shaped bacterium, designated strain MS10(T), was isolated from the surface of leaves of the black mangrove Avicennia germinans and was subjected to a polyphasic taxonomic study. Strain MS10(T) was able to grow at NaCl concentrations in the range 5-20% (w/v) with optimum growth at 10% (w/v) NaCl. Growth occurred at temperatures of 10-50 degrees C (optimal growth at 33-35 degrees C) and pH 6.0-9.0 (optimal growth at pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MS10(T) fell within the branch encompassing members of the genus Halobacillus and was most closely related to Halobacillus dabanensis JCM 12772(T) (99.2% 16S rRNA gene sequence similarity). The DNA G+C content of strain MS10(T) was 45.7 mol%, the major respiratory isoprenoid quinone was MK-7 and the cell-wall peptidoglycan was of the L-Orn-D-Asp type, characteristics consistent with its affiliation to the genus Halobacillus. Strain MS10(T) showed a level of DNA-DNA hybridization with H. dabanensis JCM 12772(T) of 29% and levels below 70% were also obtained with respect to other recognized members of the genus Halobacillus. The major fatty acids of strain MS10(T) were iso-C(16:0), anteiso-C(15:0), iso-C(14:0) and iso-C(15:0). Overall, the phenotypic, genotypic and phylogenetic results presented in this study demonstrate that strain MS10(T) represents a novel species of the genus Halobacillus, for which the name Halobacillus mangrovi sp. nov. is proposed. The type strain is MS10(T) (=CECT 7206(T)=CCM 7397(T)).
|
| 巻・号 |
58(Pt 1)
|
| ページ |
125-30
|
| 公開日 |
2008-1-1
|
| DOI |
10.1099/ijs.0.65008-0
|
| PII |
58/1/125
|
| PMID |
18175696
|
| MeSH |
Avicennia / microbiology*
Bacillaceae / classification*
Bacillaceae / genetics
Bacillaceae / isolation & purification*
Bacillaceae / physiology
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / analysis
Fatty Acids / analysis
Genes, rRNA
Genotype
Molecular Sequence Data
Nucleic Acid Hybridization
Phenotype
Phylogeny
Plant Leaves / microbiology
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sodium Chloride*
Species Specificity
|
| IF |
2.415
|
| 引用数 |
20
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM12771
JCM12772
JCM11546 |
