RRC ID 51921
Author Podyma-Inoue KA, Moriwaki T, Rajapakshe AR, Terasawa K, Hara-Yokoyama M.
Title Characterization of Heparan Sulfate Proteoglycan-positive Recycling Endosomes Isolated from Glioma Cells.
Journal Cancer Genomics Proteomics
Abstract BACKGROUND:Heparan sulfate proteoglycans (HSPGs)-dependent endocytic events have been involved in glioma progression. Thus, comprehensive understanding of the intracellular trafficking complexes formed in presence of HSPGs would be important for development of glioma treatments.
MATERIALS AND METHODS:Subcellular fractionation was used to separate vesicles containing HSPGs from the rat C6 glioma cell line. Isolated HSPG-positive vesicles were further characterized with liquid chromatography-mass spectrometry.
RESULTS:The HSPG-positive vesicular fractions, distinct from plasma membrane-derived material, were enriched in endocytic marker, Rab11. Proteomic analysis identified more than two hundred proteins to be associated with vesicular membrane, among them, over eighty were related to endosomal uptake, recycling or vesicular transport.
CONCLUSION:Part of HSPGs in glioma cells is internalized through clathrin-dependent endocytosis and undergo recycling. The development of compounds regulating HSPG-mediated trafficking will likely enable design of effective glioma treatment.
Volume 13(6)
Pages 443-452
Published 2016-11-12
DOI 10.21873/cgp.20007
PII 13/6/443
PMID 27807067
PMC PMC5219918
MeSH Animals Cell Line, Tumor Clathrin / genetics Endocytosis / genetics Endosomes / metabolism Endosomes / pathology Glioma / genetics Glioma / metabolism* Glioma / pathology Heparan Sulfate Proteoglycans / genetics Heparan Sulfate Proteoglycans / metabolism* Humans Proteomics Rats Transport Vesicles / metabolism* Transport Vesicles / pathology rab GTP-Binding Proteins / biosynthesis* rab GTP-Binding Proteins / genetics
IF 3.147
Times Cited 3
Resource
Human and Animal Cells C6(RCB2854)