RRC ID 51937
Author Okochi M, Koike S, Tanaka M, Honda H.
Title Detection of Her2-overexpressing cancer cells using keyhole shaped chamber array employing a magnetic droplet-handling system.
Journal Biosens Bioelectron
Abstract An on-chip gene expression analysis compartmentalized in droplets was developed for detection of cancer cells at a single-cell level. The chip consists of a keyhole-shaped reaction chamber with hydrophobic modification employing a magnetic bead-droplet-handling system with a gate for bead separation. Using three kinds of water-based droplets in oil, a droplet with sample cells, a lysis buffer with magnetic beads, and RT-PCR buffer, parallel magnetic manipulation and fusion of droplets were performed using a magnet-handling device containing small external magnet patterns in an array. The actuation with the magnet offers a simple system for droplet manipulation that allows separation and fusion of droplets containing magnetic beads. After reverse transcription and amplification by thermal cycling, fluorescence was obtained for detection of overexpressing genes. For clinical detection of gastric cancer cells in peritoneal washing, the Her2-overexpressing gastric cancer cells spiked within normal cells was detected by gene expression analysis of droplets containing an average of 2.5 cells. Our developed droplet-based cancer detection system manipulated by external magnetic force without pumps or valves offers a simple and flexible set-up for transcriptional detection of cancer cells, and will be greatly advantageous for less-invasive clinical diagnosis and prognostic prediction.
Volume 93
Pages 32-39
Published 2017-7-15
DOI 10.1016/j.bios.2016.11.013
PII S0956-5663(16)31159-9
PMID 27866824
MeSH Biosensing Techniques* Gene Expression Regulation, Neoplastic Humans Magnetic Fields Microfluidic Analytical Techniques / methods Prognosis Receptor, ErbB-2 / genetics Receptor, ErbB-2 / isolation & purification* Stomach Neoplasms / diagnosis* Stomach Neoplasms / genetics
IF 10.257
Times Cited 2
Human and Animal Cells RPMI1788(RCB1878)