論文 - 詳細
| RRC ID | 51953 |
|---|---|
| 著者 | Noguchi T, Ebina K, Hirao M, Morimoto T, Koizumi K, Kitaguchi K, Matsuoka H, Iwahashi T, Yoshikawa H. |
| タイトル | Oxygen ultra-fine bubbles water administration prevents bone loss of glucocorticoid-induced osteoporosis in mice by suppressing osteoclast differentiation. |
| ジャーナル | Osteoporos Int |
| Abstract |
Oxygen ultra-fine bubbles (OUB) saline injection prevents bone loss of glucocorti\coid-induced osteoporosis in mice, and OUB inhibit osteoclastogenesis via RANK-TRAF6-c-Fos-NFATc1 signaling and RANK-p38 MAPK signaling in vitro. INTRODUCTION:Ultra-fine bubbles (<200 nm in diameter) have several unique properties, and they are tested in various medical fields. The purpose of this study was to investigate the effects of oxygen ultra-fine bubbles (OUB) on glucocorticoid-induced osteoporosis (GIO) model mice. METHODS:Prednisolone (PSL, 5 mg) was subcutaneously inserted in 6-month-old male C57BL/6J mice, and 200 μl of saline, OUB-diluted saline, or nitrogen ultra-fine bubbles (NUB)-diluted saline was intraperitoneally injected three times per week for 8 weeks the day after operations. Mice were divided into four groups; (1) control, sham-operation + saline; (2) GIO, PSL + saline; (3) GIO + OUB, PSL + OUB saline; (4) GIO + NUB, PSL + NUB saline. The effects of OUB on osteoblasts and osteoclasts were examined by serially diluted OUB medium in vitro. RESULTS:Bone mass was significantly decreased in GIO [bone volume/total volume (%): control vs. GIO 12.6 vs. 7.9; p < 0.01] while significantly preserved in GIO + OUB (GIO vs. GIO + OUB 7.9 vs. 12.9; p < 0.05). In addition, tartrate-resistant acid phosphatase (TRAP)-positive cells in the distal femur [mean osteoclasts number/bone surface (mm-1)] was significantly increased in GIO (control vs. GIO 6.8 vs. 11.6; p < 0.01) while suppressed in GIO + OUB (GIO vs. GIO + OUB 11.6 vs. 7.5; p < 0.01). NUB did not affect these parameters. In vitro experiments revealed that OUB significantly inhibited osteoclastogenesis by inhibiting RANK-TRAF6-c-Fos-NFATc1 signaling, RANK-p38 MAPK signaling, and TRAP/Cathepsin K/DC-STAMP mRNA expression in a concentration-dependent manner. OUB did not affect osteoblastogenesis in vitro. CONCLUSIONS:OUB prevent bone loss in GIO mice by inhibiting osteoclastogenesis. |
| 巻・号 | 28(3) |
| ページ | 1063-1075 |
| 公開日 | 2017-3-1 |
| DOI | 10.1007/s00198-016-3830-1 |
| PII | 10.1007/s00198-016-3830-1 |
| PMID | 27896363 |
| MeSH | Animals Bone Density / drug effects Bone Remodeling / drug effects Cell Differentiation / drug effects Cells, Cultured Disease Models, Animal Glucocorticoids Humans Male Mice, Inbred C57BL Microbubbles Nanoparticles Osteoblasts / drug effects Osteoclasts / cytology Osteoclasts / drug effects* Osteogenesis / drug effects Osteoporosis / chemically induced Osteoporosis / prevention & control* Oxygen / administration & dosage Oxygen / therapeutic use* Prednisolone |
| IF | 3.857 |
| 引用数 | 6 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |