RRC ID 51958
Author Utsunomiya T, Ishibazawa A, Nagaoka T, Hanada K, Yokota H, Ishii N, Yoshida A.
Title Transforming Growth Factor-β Signaling Cascade Induced by Mechanical Stimulation of Fluid Shear Stress in Cultured Corneal Epithelial Cells.
Journal Invest Ophthalmol Vis Sci
Abstract Purpose:Because blinking is regarded as mechanical stimulation of fluid shear stress on the corneal epithelial cells, we investigated the effects of fluid shear stress on cultured human corneal epithelial cells (HCECs).
Methods:The HCECs were exposed to shear stress (0, 1.2, 12 dyne/cm2) with the parallel-plate type of flow chamber. Wound healing, cellular proliferation, growth factor expression, TGF-β1 concentration in the culture supernatant, and phosphorylation of SMAD2 were investigated.
Results:Monolayers of HCECs exposed to shear stress had delayed wound healing and decreased proliferation compared with those of the static control (0 dyne/cm2). With increasing shear stress, TGF-β1 expression and phosphorylation of SMAD2 increased significantly, but the levels of total TGF-β1 in the culture supernatant decreased significantly. Delayed wound healing, decreased proliferation, and phosphorylation of the SMAD2 by shear stress were canceled out with a TGF-β receptor inhibitor.
Conclusions:Fluid shear stress on the HCECs affected TGF-β signaling, which was associated with delayed wound healing. Mechanical stress by blinking might involve TGF-β signaling, and activation of TGF-β might be a key factor in wound healing of the corneal epithelium. Further studies should investigate the molecular mechanism of shear stress-induced activation of TGF-β.
Volume 57(14)
Pages 6382-6388
Published 2016-11-1
DOI 10.1167/iovs.16-20638
PII 2589780
PMID 27898984
MeSH Blotting, Western Cell Proliferation Cells, Cultured Corneal Injuries / genetics Corneal Injuries / metabolism* Corneal Injuries / pathology Enzyme-Linked Immunosorbent Assay Epithelium, Corneal / metabolism* Epithelium, Corneal / pathology Gene Expression Regulation* Humans Phosphorylation RNA / genetics* Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Smad2 Protein / metabolism* Stress, Mechanical Transforming Growth Factor beta / biosynthesis Transforming Growth Factor beta / genetics* Wound Healing / physiology
IF 3.47
Times Cited 6
Human and Animal Cells HCE-T(RCB2280)