RRC ID 51978
Author Li S, Gao A, Dong S, Chen Y, Sun S, Lei Z, Zhang Z.
Title Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.
Journal Int J Biol Macromol
Abstract Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence. Three main fractions MP-1, MP-3 and MP-4 were obtained during the purification steps. The recovery rates based on MPS used were 26.2%, 29.1% and 18.7% for MP-1, MP-3 and MP-4 respectively. The monosaccharide composition, ultraviolet spectrum, infrared spectrum and NMR of the three fractions were analyzed. Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated. The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL. The fractions of MP-1, MP-3 and MP-4 induced apoptosis in HepG-2 cells and Hela cells by arresting cell cycle progression at the G0/G1 phase. These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.
Volume 96
Pages 26-34
Published 2017-3-1
DOI 10.1016/j.ijbiomac.2016.12.007
PII S0141-8130(16)31342-3
PMID 27939510
MeSH Animals Antineoplastic Agents / chemistry Antineoplastic Agents / isolation & purification Antineoplastic Agents / pharmacology Ascomycota / metabolism* Cell Cycle / drug effects Cell Proliferation / drug effects Fermentation* HeLa Cells Hep G2 Cells Humans Immunologic Factors / chemistry Immunologic Factors / isolation & purification Immunologic Factors / pharmacology Macrophages / cytology Macrophages / drug effects Macrophages / immunology Macrophages / metabolism Mice Monosaccharides / analysis Nitric Oxide / biosynthesis Phagocytosis / drug effects Polysaccharides / chemistry Polysaccharides / isolation & purification* Polysaccharides / pharmacology* RAW 264.7 Cells Soybeans / chemistry* Soybeans / metabolism Soybeans / microbiology*
IF 5.162
Times Cited 36
Human and Animal Cells Hep G2(RCB1886) HeLa(RCB3680)