RRC ID 52034
Author Shinoda Y, Takahashi T, Akimoto J, Ichikawa M, Yamazaki H, Narumi A, Yano S, Fujiwara Y.
Title Comparative photodynamic therapy cytotoxicity of mannose-conjugated chlorin and talaporfin sodium in cultured human and rat cells.
Journal J Toxicol Sci
Abstract Photodynamic therapy (PDT) is a Food and Drug Administration authorized method for cancer treatment, which uses photosensitizer and laser photo-irradiation to generate reactive oxygen species to induce cell death in tumors. Photosensitizers have been progressively developed, from first to third generation, with improvements in cell specificity, reduced side effects and toxicity, increased sensitivity for irradiation and reduced persistence of photosensitizer in healthy cells. These improvements have been achieved by basic comparative experiments between current and novel photosensitizers using cell lines; however, photosensitizers should be carefully evaluated because they may have cell type specificity. In the present study, we compared a third-generation photosensitizer, β-mannose-conjugated chlorin (β-M-chlorin), with the second generation, talaporfin sodium (NPe6), using seven different rat and human cell lines and a neuronal/glial primary culture prepared from rat embryos. NPe6 was more effective than β-M-chlorin in human-derived cell lines, and β-M-chlorin was more effective than NPe6 in rat primary cultures and rat-derived cell lines, except for the rat pheochromocytoma cell line, PC12. These differences of phototoxicity in different cell types are not because of differences in photosensitivity between the photosensitizers, but rather are associated with different distribution and accumulation rates in the different cell types. These data suggest that evaluation of photosensitizers for PDT should be carried out using as large a variety of cell types as possible because each photosensitizer may have cell type specificity.
Volume 42(1)
Pages 111-119
Published 2017-1-1
DOI 10.2131/jts.42.111
PMID 28070104
MeSH Animals Antineoplastic Agents / pharmacology* Cell Line Cell Line, Tumor Cell Survival / drug effects Cell Survival / radiation effects Cells, Cultured Humans Light* Mannose / chemistry Mannose / pharmacology* Neurons / drug effects Neurons / metabolism Neurons / radiation effects PC12 Cells Photochemotherapy* Photosensitizing Agents / pharmacology* Porphyrins / chemistry Porphyrins / pharmacology* Rats Rats, Wistar
IF 1.737
Times Cited 3
Resource
Human and Animal Cells T98G(RCB1954)