| RRC ID |
52058
|
| 著者 |
Otaka A, Kitagawa K, Nakaoki T, Hirata M, Fukazawa K, Ishihara K, Mahara A, Yamaoka T.
|
| タイトル |
Label-Free Separation of Induced Pluripotent Stem Cells with Anti-SSEA-1 Antibody Immobilized Microfluidic Channel.
|
| ジャーナル |
Langmuir
|
| Abstract |
When induced pluripotent stem cells (iPSCs) are routinely cultured, the obtained cells are a heterogeneous mixture, including feeder cells and partially differentiated cells. Therefore, a purification process is required to use them in a clinical stage. We described a label-free separation of iPSCs using a microfluidic channel. Antibodies against stage-specific embryonic antigen 1 (SSEA-1) was covalently immobilized on the channel coated with a phospholipid polymer. After injection of the heterogeneous cell suspension containing iPSCs, the velocity of cell movement under a liquid flow condition was measured. The mean velocity of the cell movement was 2.1 mm/sec in the unmodified channel, while that in the channel with the immobilized-antibody was 0.4 mm/sec. The eluted cells were fractionated by eluting time. As a result, the SSEA-1 positive iPSCs were mainly contained in later fractions, and the proportion of iPSCs was increased from 43% to 82% as a comparison with the initial cell suspension. These results indicated that iPSCs were selectively separated by the microfluidic channel. This channel is a promising device for label-free separation of iPSCs based on their pluripotent state.
|
| 巻・号 |
33(6)
|
| ページ |
1576-1582
|
| 公開日 |
2017-2-14
|
| DOI |
10.1021/acs.langmuir.6b04070
|
| PMID |
28092949
|
| MeSH |
Animals
Antibodies, Monoclonal / chemistry*
Cell Separation / methods*
Induced Pluripotent Stem Cells / cytology*
Lewis X Antigen / immunology*
Methacrylates / chemical synthesis
Methacrylates / chemistry
Mice
Microfluidic Analytical Techniques / methods*
Polyethylene Glycols / chemical synthesis
Polyethylene Glycols / chemistry
Polymethacrylic Acids / chemistry
|
| IF |
3.557
|
| 引用数 |
6
|
| リソース情報 |
| ヒト・動物細胞 |
iPS-MEF-Ng-178B-5(APS0002) |
