RRC ID 52067
Author Sato M, Kawana K, Adachi K, Fujimoto A, Taguchi A, Fujikawa T, Yoshida M, Nakamura H, Nishida H, Inoue T, Ogishima J, Eguchi S, Yamashita A, Tomio K, Arimoto T, Wada-Hiraike O, Oda K, Nagamatsu T, Osuga Y, Fujii T.
Title Low uptake of fluorodeoxyglucose in positron emission tomography/computed tomography in ovarian clear cell carcinoma may reflect glutaminolysis of its cancer stem cell-like properties.
Journal Oncol Rep
Abstract The characteristics of ovarian cancers that showed low activation of glycolysis were investigated. Using medical records of patients with ovarian cancers who had undergone fluorodeoxyglucose positron emission tomography/computed tomography (FDG-PET/CT) prior to their primary surgery at the University of Tokyo Hospital between 2010 and 2015, we identified cases with a low uptake of FDG in PET/CT. We considered the maximum standardized uptake value (SUVmax) as the degree of glucose uptake. We investigated the properties which may account for the low activation of glycolysis in vitro. The expression level of alanine, serine, cysteine-preferring transporter 2 (ASCT2, a glutamine influx transporter), system L-type amino acid transporter 1 (LAT1, a glutamine efflux transporter) and glucose transporter 1 (GLUT1, a glucose influx transporter) were investigated by western blotting. The phosphorylation level of AMP-activated protein kinase (AMPK), which is one of the metabolic sensors, was also investigated. Most of the cases with a low uptake SUVmax were limited to patients with ovarian clear cell carcinoma (CCC). We obtained cancer stem cell (CSC)-like properties from CCC cell lines, and compared the expression levels of transporters between non-CSCs and CSCs. Whereas the expression level of ASCT2 was nearly unchanged between non-CSCs and CSCs, the expression levels of LAT1 and GLUT1 were decreased in CSCs compared to non-CSCs. The phosphorylation level of AMPK was reduced in CSCs compared to non-CSCs. In conclusion, we suggested that ovarian CCC showed low activation of glycolysis, and this may reflect glutaminolysis of its CSC-like properties.
Volume 37(3)
Pages 1883-1888
Published 2017-3-1
DOI 10.3892/or.2017.5398
PMID 28112360
MeSH Adenocarcinoma, Clear Cell / diagnostic imaging Adenocarcinoma, Clear Cell / metabolism Adenocarcinoma, Clear Cell / pathology Adenocarcinoma, Mucinous / diagnostic imaging Adenocarcinoma, Mucinous / metabolism Adenocarcinoma, Mucinous / pathology Adult Aged Blotting, Western Cystadenocarcinoma, Serous / diagnostic imaging Cystadenocarcinoma, Serous / metabolism Cystadenocarcinoma, Serous / pathology Endometrial Neoplasms / diagnostic imaging Endometrial Neoplasms / metabolism Endometrial Neoplasms / pathology Female Fluorodeoxyglucose F18* Glutamine / metabolism* Humans Middle Aged Neoplasm Staging Neoplastic Stem Cells / metabolism Neoplastic Stem Cells / pathology* Ovarian Neoplasms / diagnostic imaging Ovarian Neoplasms / metabolism Ovarian Neoplasms / pathology* Positron Emission Tomography Computed Tomography / methods* Prognosis Radiopharmaceuticals Signal Transduction Tumor Cells, Cultured Young Adult
IF 3.041
Times Cited 3
Human and Animal Cells JHOC-5(RCB1520)