Reference - Detail
| RRC ID | 52090 |
|---|---|
| Author | Hang Q, Isaji T, Hou S, Wang Y, Fukuda T, Gu J. |
| Title | A Key Regulator of Cell Adhesion: Identification and Characterization of Important N-Glycosylation Sites on Integrin α5 for Cell Migration. |
| Journal | Mol Cell Biol |
| Abstract |
The N-glycosylation of integrin α5β1 is thought to control many fundamental aspects of cell behavior, including cell adhesion and migration. However, the mechanism of how N-glycans function remains largely obscure. Here, we used a loss-of-function approach. Wild-type (WT) integrin α5 and N-glycosylation mutant S3-5 (sites 3 to 5) integrin α5, which contains fewer N-glycans, were stably reconstituted in α5 knockout cancer cells. We found that the migration ability of S3-5 cells was decreased in comparison with that of the WT. Interestingly, the levels of phosphorylated focal adhesion kinase and actin stress fiber formation were greatly enhanced in the S3-5 mutant. In a mechanistic manner, the internalization of active but not total integrin α5β1 was inhibited in S3-5 cells, which is a process that is related to the enhanced expression of active integrin α5β1 on the cell surface. Importantly, restoration of N-glycosylation on the β-propeller domain of α5 reinstated the cell migration ability, active α5β1 expression, and internalization. Moreover, these N-glycans are critical for α5-syndecan-4 complex formation. These findings indicate that N-glycosylation on the β-propeller domain functions as a molecular switch to control the dynamics of α5β1 on the cell surface that in turn is required for optimum adhesion for cell migration. |
| Volume | 37(9) |
| Published | 2017-5-1 |
| DOI | 10.1128/MCB.00558-16 |
| PII | MCB.00558-16 |
| PMID | 28167607 |
| PMC | PMC5394273 |
| MeSH | Cell Adhesion / physiology* Cell Line, Tumor Cell Membrane / metabolism* Cell Movement / physiology* Focal Adhesion Kinase 1 / metabolism Glycosylation HEK293 Cells HeLa Cells Humans Integrin alpha5beta1 / metabolism* Multiprotein Complexes / metabolism Phosphorylation Protein Structure, Tertiary Syndecan-4 / metabolism |
| IF | 3.611 |
| Times Cited | 17 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 293T(RCB2202) HeLa |