| RRC ID |
52099
|
| 著者 |
Yoshida J, Uesugi S, Kawamura T, Kimura KI, Hu D, Xia S, Toyooka N, Ohnishi M, Kawashima H.
|
| タイトル |
(4Z,15Z)-Octadecadienoic Acid Inhibits Glycogen Synthase Kinase-3β and Glucose Production in H4IIE Cells.
|
| ジャーナル |
Lipids
|
| Abstract |
Many uncommon non-methylene-interrupted fatty acids (NMI FA) are present in limpet gonads, but their biological properties remain unknown. To investigate new biological effects of naturally occurring NMI FA in eukaryotic cells, the biological activities of structurally analogous (4Z,15Z)-octadecadienoic acid (1), (9Z,20Z)-tricosadienoic acid (2), and (12Z,23Z)-hexacosadienoic acid (3) were examined by using a yeast-based drug-screening system using the Ca2+-sensitive mutant strain, Saccharomyces cerevisiae (zds1Δ erg3Δ pdr1Δ pdr3Δ). Among 1-3, 1 showed restored growth activity at a dose of 80 µg/disc in the mutant yeast strain. This phenotype suggests that 1 suppresses Ca2+-signaling of the mutant yeast through inhibition of glycogen synthase kinase-3β (GSK-3β) or calcineurin pathways or both. From this result, the inhibitory activity of 1-3 against GSK-3β was further determined. 1-3 showed potent inhibitory activity against GSK-3β with IC50 values ranging from 8.7 to 21.9 µM. Inhibition of GSK-3β reduces gene expression of the gluconeogenic key enzymes in liver, so we analyzed glucose production in rat hepatoma H4IIE cells to assess GSK-3β inhibitory activity of 1-3. Acid 1 inhibited glucose production at 25 µM in H4IIE cells. Our results would open up new possibilities for an anti-diabetic effect of 1 and might provide important insights into understanding the biological properties of naturally occurring NMI FA.
|
| 巻・号 |
52(3)
|
| ページ |
295-301
|
| 公開日 |
2017-3-1
|
| DOI |
10.1007/s11745-017-4236-3
|
| PII |
10.1007/s11745-017-4236-3
|
| PMID |
28194557
|
| MeSH |
Animals
Calcineurin / metabolism
Calcium / metabolism
Cell Line
Cell Survival / drug effects
Drug Evaluation, Preclinical
Fatty Acids, Unsaturated / chemistry
Fatty Acids, Unsaturated / pharmacology*
Glucose / metabolism*
Glycogen Synthase Kinase 3 beta / metabolism*
HL-60 Cells
Humans
Rats
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / growth & development*
|
| IF |
1.919
|
| 引用数 |
6
|
| リソース情報 |
| ヒト・動物細胞 |
HL60(RCB0041)
WI-38(RCB0702) |
