| RRC ID |
52102
|
| 著者 |
Wang CL, Wang H, Xiao F, Wang CD, Hu GL, Zhu JF, Shen C, Zuo B, Cui YM, Li D, Yuan-Gao, Zhang XL, Chen XD.
|
| タイトル |
Cyclic compressive stress-induced scinderin regulates progress of developmental dysplasia of the hip.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Developmental dysplasia of the hip (DDH) is a common musculoskeletal disorder characterized by a mismatch between acetabulum and femoral head. Mechanical force plays an important role during the occurrence and development of abnormities in acetabulum and femoral head. In this study, we established a mechanical force model named cyclic compressive stress (Ccs). To analyze the effect of Ccs on DDH, we detected special genes in chondrocytes and osteoblasts. Results showed that Ccs downregulated chondrogenesis of ADTC5 in a concentration-dependent manner. Moreover, the mRNA level of Scinderin (Scin) considerably increased. We established lentivirus-SCIN(GV144-SCIN) to transfect hBMSCs, which were treated with different Ccs levels (0.25 Hz*5 cm, 0.5 Hz*5 cm, and 1 Hz*10 cm); the result showed that overexpression of Scin upregulated osteogenesis and osteoclastogenesis. By contrast, expression of chondrocyte-specific genes, including ACAN, COL-2A, and Sox9, decreased. Further molecular investigation demonstrated that Scin promoted osteogenesis and osteoclastogenesis through activation of the p-Smad1/5/8, NF-κB, and MAPK P38 signaling pathways, as well as stimulated the expression of key osteoclast transcriptional factors NFATc1 and c-Fos. Moreover, Scin-induced osteogenesis outweighed osteoclastogenesis in defective femur in vivo. The results of the analysis of Micro-CT confirmed these findings. Overall, Ccs influenced the development of DDH by promoting osteogenesis and cartilage degradation. In addition, Scin played a vital role in the development of DDH.
|
| 巻・号 |
485(2)
|
| ページ |
400-408
|
| 公開日 |
2017-4-1
|
| DOI |
10.1016/j.bbrc.2017.02.065
|
| PII |
S0006-291X(17)30335-2
|
| PMID |
28213129
|
| MeSH |
Animals
Blotting, Western
Cell Line, Tumor
Cells, Cultured
Chondrocytes / metabolism
Chondrogenesis / genetics
Disease Progression
Gelsolin / genetics*
Gelsolin / metabolism
Gene Expression Regulation*
Hip Dislocation, Congenital / genetics*
Hip Dislocation, Congenital / metabolism
Hip Dislocation, Congenital / pathology
Humans
MAP Kinase Signaling System
Mesenchymal Stem Cell Transplantation
Mesenchymal Stem Cells / metabolism
Mice, Nude
NF-kappa B / metabolism
Osteoblasts / metabolism
Osteogenesis / genetics
Rats
Reverse Transcriptase Polymerase Chain Reaction
Stress, Mechanical*
Transplantation, Heterologous
|
| IF |
2.985
|
| 引用数 |
6
|
| リソース情報 |
| ヒト・動物細胞 |
ATDC5(RCB0565) |
