| RRC ID |
52111
|
| Author |
Tsukune N, Naito M, Kubota T, Ozawa Y, Nagao M, Ohashi A, Sato S, Takahashi T.
|
| Title |
Lamin A overexpression promotes osteoblast differentiation and calcification in the MC3T3-E1 preosteoblastic cell line.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Lamin A/C is a component of the nuclear lamina, which is involved in cellular proliferation and differentiation. However, the mechanism by which lamin A regulates osteoblast differentiation is not well understood. In this study, we investigated lamin A/C expression during osteoblast differentiation in a preosteoblastic cell line, MC3T3-E1. Real-time PCR analysis showed that lamin A/C mRNA expression was upregulated during BMP-2 induced osteoblast differentiation. Treatment with the estrogen receptor antagonist, fulvestrant, inhibited osteoblast differentiation and the upregulation of lamin A/C mRNA and protein expressions in the presence of BMP-2. These results clearly demonstrated that lamin A/C expression correlates with osteoblast differentiation. To determine the roles of lamin A expression in osteoblast differentiation, MC3T3-E1 cells were transfected with a vector overexpressing lamin A. Results showed that lamin A overexpression promoted osteoblast differentiation and calcification by inducing the expression of alkaline phosphatase, type 1 collagen, BSP, osteocalcin, and DMP-1 in the presence of BMP-2. Furthermore, lamin A overexpression partially restored osteoblastic capacity in the presence of fulvestrant by increasing the expression of BSP, osteocalcin, and DMP-1. These results suggest that lamin A plays important roles in maintaining the osteoblast differentiation and function.
|
| Volume |
488(4)
|
| Pages |
664-670
|
| Published |
2017-7-8
|
| DOI |
10.1016/j.bbrc.2017.02.110
|
| PII |
S0006-291X(17)30401-1
|
| PMID |
28237702
|
| MeSH |
Animals
Calcification, Physiologic*
Cell Differentiation*
Cells, Cultured
Lamin Type A / genetics*
Lamin Type A / metabolism
Mice
Mice, Inbred C57BL
Osteoblasts / cytology*
Osteoblasts / metabolism*
|
| IF |
2.985
|
| Times Cited |
13
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
