RRC ID 52120
著者 Takeuchi T, Sugimoto A, Imazato N, Tamura M, Nakatani S, Kobata K, Arata Y.
タイトル Glucosamine Suppresses Osteoclast Differentiation through the Modulation of Glycosylation Including O-GlcNAcylation.
ジャーナル Biol Pharm Bull
Abstract Osteoclasts represent the only bone resorbing cells in an organism. In this study, we investigated the effect of glucosamine (GlcN), a nutrient used to prevent joint pain and bone loss, on the osteoclastogenesis of murine macrophage-like RAW264 cells. GlcN supplementation suppressed the upregulation of osteoclast-specific genes (tartrate-resistant acid phosphatase (TRAP), cathepsin K, matrix metallopeptidase 9, and nuclear factor of activated T cell c1 (NFATc1)), receptor activator of nuclear factor-κB ligand (RANKL)-dependent upregulation of TRAP enzyme activity, and the formation of TRAP-positive multinuclear cells more effectively than N-acetylglucosamine (GlcNAc), which we have previously shown to inhibit osteoclast differentiation. To clarify the mechanism by which GlcN suppresses osteoclastogenesis, we further investigated the effect of GlcN on O-GlcNAcylation by Western blotting and on other types of glycosylation by lectin blotting. We found that, upon addition of GlcN, the O-GlcNAcylation of cellular proteins was increased whereas α2,6-linked sialic acid modification was decreased. Therefore, these glycan modifications in cellular proteins may contribute to the suppression of osteoclastogenesis.
巻・号 40(3)
ページ 352-356
公開日 2017-1-1
DOI 10.1248/bpb.b16-00877
PMID 28250278
MeSH Acylation Animals Bone Resorption / metabolism Cell Differentiation / drug effects* Cell Line Glucosamine / pharmacology* Glycosylation Macrophages / drug effects Macrophages / metabolism Mice Osteoclasts / drug effects* Osteoclasts / metabolism Osteogenesis / drug effects* Protein Processing, Post-Translational / drug effects* RANK Ligand / metabolism Tartrate-Resistant Acid Phosphatase / metabolism Up-Regulation
IF 1.863
引用数 3
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)