論文 - 詳細
| RRC ID | 52142 |
|---|---|
| 著者 | Nguyen TD, Takasuka H, Kaku Y, Inoue S, Nagamune T, Kawahara M. |
| タイトル | Engineering a growth sensor to select intracellular antibodies in the cytosol of mammalian cells. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
Intracellular antibodies (intrabodies) are expected to function as therapeutics as well as tools for elucidating in vivo function of proteins. In this study, we propose a novel intrabody selection method in the cytosol of mammalian cells by utilizing a growth signal, induced by the interaction of the target antigen and an scFv-c-kit growth sensor. Here, we challenge this method to select specific intrabodies against rabies virus nucleoprotein (RV-N) for the first time. As a result, we successfully select antigen-specific intrabodies from a naïve synthetic library using phage panning followed by our growth sensor-based intracellular selection method, demonstrating the feasibility of the method. Additionally, we succeed in improving the response of the growth sensor by re-engineering the linker region of its construction. Collectively, the described selection method utilizing a growth sensor may become a highly efficient platform for selection of functional intrabodies in the future. |
| 巻・号 | 124(1) |
| ページ | 125-132 |
| 公開日 | 2017-7-1 |
| DOI | 10.1016/j.jbiosc.2017.02.017 |
| PII | S1389-1723(17)30004-X |
| PMID | 28319021 |
| MeSH | Animals Antibodies / genetics* Antibodies / immunology Cytosol / metabolism* Nucleoproteins / immunology Peptide Library Protein Engineering / methods* Rabies virus |
| IF | 2.366 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | Ba/F3(RCB0805) |