論文 - 詳細
| RRC ID | 52150 |
|---|---|
| 著者 | Chen YM, Chen LH, Li MP, Li HF, Higuchi A, Kumar SS, Ling QD, Alarfaj AA, Munusamy MA, Chang Y, Benelli G, Murugan K, Umezawa A. |
| タイトル | Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs. |
| ジャーナル | Sci Rep |
| Abstract |
Establishing cultures of human embryonic (ES) and induced pluripotent (iPS) stem cells in xeno-free conditions is essential for producing clinical-grade cells. Development of cell culture biomaterials for human ES and iPS cells is critical for this purpose. We designed several structures of oligopeptide-grafted poly (vinyl alcohol-co-itaconic acid) hydrogels with optimal elasticity, and prepared them in formations of single chain, single chain with joint segment, dual chain with joint segment, and branched-type chain. Oligopeptide sequences were selected from integrin- and glycosaminoglycan-binding domains of the extracellular matrix. The hydrogels grafted with vitronectin-derived oligopeptides having a joint segment or a dual chain, which has a storage modulus of 25 kPa, supported the long-term culture of human ES and iPS cells for over 10 passages. The dual chain and/or joint segment with cell adhesion molecules on the hydrogels facilitated the proliferation and pluripotency of human ES and iPS cells. |
| 巻・号 | 7 |
| ページ | 45146 |
| 公開日 | 2017-3-23 |
| DOI | 10.1038/srep45146 |
| PII | srep45146 |
| PMID | 28332572 |
| PMC | PMC5362828 |
| MeSH | Cell Differentiation / genetics Cells, Cultured Embryonic Stem Cells / cytology Embryonic Stem Cells / metabolism Humans Hydrogels / chemistry* Oligopeptides / metabolism* Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / metabolism* Protein Binding Protein Interaction Domains and Motifs |
| IF | 3.998 |
| 引用数 | 53 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 6.0 |
| リソース情報 | |
| ヒト・動物細胞 | 454E2(HPS0077) |