RRC ID 52151
著者 Ikeda Y, Morita SY, Terada T.
タイトル Cholesterol attenuates cytoprotective effects of phosphatidylcholine against bile salts.
ジャーナル Sci Rep
Abstract Bile salts have potent detergent properties and damaging effects on cell membranes, leading to liver injury. However, the molecular mechanisms for the protection of hepatocytes against bile salts are not fully understood. In this study, we demonstrated that the cytotoxicity of nine human major bile salts to HepG2 cells and primary human hepatocytes was prevented by phosphatidylcholine (PC). In contrast, cholesterol had no direct cytotoxic effects but suppressed the cytoprotective effects of PC. PC reduced the cell-association of bile salt, which was reversed by cholesterol. Light scattering measurements and gel filtration chromatography revealed that cholesterol within bile salt/PC dispersions decreased mixed micelles but increased vesicles, bile salt simple micelles and monomers. These results suggest that cholesterol attenuates the cytoprotective effects of PC against bile salts by facilitating the formation of bile salt simple micelles and monomers. Therefore, biliary PC and cholesterol may play different roles in the pathogenesis of bile salt-induced liver injury.
巻・号 7(1)
ページ 306
公開日 2017-3-22
DOI 10.1038/s41598-017-00476-2
PII 10.1038/s41598-017-00476-2
PMID 28331225
PMC PMC5428433
MeSH Bile Acids and Salts / metabolism* Cholesterol / metabolism* Chromatography, Gel Dynamic Light Scattering Gastrointestinal Agents / metabolism* Hep G2 Cells Hepatocytes / drug effects* Hepatocytes / physiology* Humans Phosphatidylcholines / metabolism*
IF 3.998
引用数 9
リソース情報
ヒト・動物細胞 Hep G2