| RRC ID |
52171
|
| 著者 |
Kasai K, Kimura Y, Miyata S.
|
| タイトル |
Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates.
|
| ジャーナル |
Mater Sci Eng C Mater Biol Appl
|
| Abstract |
Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.
|
| 巻・号 |
78
|
| ページ |
354-361
|
| 公開日 |
2017-9-1
|
| DOI |
10.1016/j.msec.2017.04.021
|
| PII |
S0928-4931(16)32266-4
|
| PMID |
28575995
|
| MeSH |
Animals
Cell Adhesion
Cell Culture Techniques
Cell Differentiation
Cell Proliferation*
Cells, Cultured
Embryonic Stem Cells
Feeder Cells
Mice
Mouse Embryonic Stem Cells
Ozone
Pluripotent Stem Cells
|
| IF |
5.88
|
| 引用数 |
7
|
| リソース情報 |
| ヒト・動物細胞 |
EB3(AES0139) |
