RRC ID 52188
著者 Tsunaka M, Shinki H, Koyama T.
タイトル Cell-based evaluation of changes in coagulation activity induced by antineoplastic drugs for the treatment of acute myeloid leukemia.
ジャーナル PLoS One
Abstract Idarubicin (IDR), cytarabine (AraC), and tamibarotene (Am80) are effective for treatment of acute myeloid leukemia (AML). In acute leukemia, the incidence of venous thromboembolism or disseminated intravascular coagulation is associated with induction chemotherapy. Procoagulant effects of IDR, AraC, and Am80 were investigated in a vascular endothelial cell line EAhy926 and AML cell lines HL60 (AML M2), NB4 (AML M3, APL), and U937 (AML M5), focusing on tissue factor (TF), phosphatidylserine (PS), and thrombomodulin (TM). IDR induced procoagulant activity on the surface of vascular endothelial and AML cell lines. Expression of TF antigen, TM antigen, and PS were induced by IDR on the surface of each cell line, whereas expression of TF and TM mRNAs were unchanged. Conversely, Am80 decreased TF exposure and procoagulant activity, and increased TM exposure on NB4 cells. In NB4 cells, we observed downregulation of TF mRNA and upregulation of TM mRNA. These data suggest IDR may induce procoagulant activity in vessels by apoptosis through PS exposure and/or TF expression on vascular endothelial and AML cell lines. Am80 may suppress blood coagulation through downregulation of TF expression and induction of TM expression. Our methods could be useful to investigate changes in procoagulant activity induced by antineoplastic drugs.
巻・号 12(4)
ページ e0175765
公開日 2017-4-13
DOI 10.1371/journal.pone.0175765
PII PONE-D-17-00289
PMID 28406995
PMC PMC5391104
MeSH Antineoplastic Agents / adverse effects* Antineoplastic Agents / pharmacology Benzoates / adverse effects Benzoates / pharmacology Cell Line, Tumor Cytarabine / adverse effects Cytarabine / pharmacology Gene Expression Regulation, Neoplastic / drug effects HL-60 Cells Humans Idarubicin / adverse effects Idarubicin / pharmacology Leukemia, Myeloid, Acute / drug therapy Leukemia, Myeloid, Acute / genetics Leukemia, Myeloid, Acute / metabolism* Phosphatidylserines / metabolism* Tetrahydronaphthalenes / adverse effects Tetrahydronaphthalenes / pharmacology Thrombomodulin / genetics Thrombomodulin / metabolism* Thromboplastin / genetics Thromboplastin / metabolism*
IF 2.74
引用数 4
リソース情報
ヒト・動物細胞 HL60 U937