| RRC ID |
52189
|
| 著者 |
Sato Y, Koshizuka T, Ishibashi K, Hashimoto K, Ishioka K, Ikuta K, Yokota SI, Fujii N, Suzutani T.
|
| タイトル |
Involvement of herpes simplex virus type 1 UL13 protein kinase in induction of SOCS genes, the negative regulators of cytokine signaling.
|
| ジャーナル |
Microbiol Immunol
|
| Abstract |
The suppressor of cytokine signaling (SOCS) family has eight members and suppresses various cytokine signaling pathways, including IFN signaling. Therefore, some viruses have evolved molecular mechanisms for inducing SOCS proteins and thus escaping host immunity. Herpes simplex virus type 1 (HSV-1) has a mechanism for escaping from type I IFN by induction of both SOCS1 and SOCS3. In this study, expression of the eight members of the SOCS family stimulated by HSV-1 infection was comparatively analyzed by qRT-PCR. It was found that SOCS1 and SOCS3 are induced by HSV-1-infection at 4 hr post infection. However, such induction was not observed in UL13 deficient virus-infected cells, suggesting that UL13 protein kinase participates in induction of both genes. The transcription factor Sp1-binding sites of SOCS3 promoter/enhancer region were identified as the regulatory elements for induction of SOCS3 in HSV-1 infected cells. Accumulation of activated Sp1 was detectable in the nuclei of HSV-1-infected cells before induction of SOCS3. Taken together, these results suggest that HSV-1 has a potent mechanism for escaping from the IFN system.
|
| 巻・号 |
61(5)
|
| ページ |
159-167
|
| 公開日 |
2017-5-1
|
| DOI |
10.1111/1348-0421.12483
|
| PMID |
28419615
|
| MeSH |
Animals
Binding Sites / genetics
Cell Line
Chlorocebus aethiops
Herpesvirus 1, Human / genetics*
Humans
Immune Evasion / genetics
Immune Evasion / immunology*
Interferon Type I / metabolism*
Promoter Regions, Genetic / genetics
Protein Kinases / genetics*
Regulatory Elements, Transcriptional / genetics
Signal Transduction / genetics
Sp1 Transcription Factor / metabolism
Suppressor of Cytokine Signaling 1 Protein / genetics
Suppressor of Cytokine Signaling 1 Protein / metabolism*
Suppressor of Cytokine Signaling 3 Protein / genetics
Suppressor of Cytokine Signaling 3 Protein / metabolism*
Vero Cells
|
| IF |
1.566
|
| 引用数 |
7
|
| リソース情報 |
| ヒト・動物細胞 |
Vero(RCB0001) |
