論文 - 詳細
| RRC ID | 52278 |
|---|---|
| 著者 | Bay DH, Busch A, Lisdat F, Iida K, Ikebukuro K, Nagasawa K, Karube I, Yoshida W. |
| タイトル | Identification of G-quadruplex structures that possess transcriptional regulating functions in the Dele and Cdc6 CpG islands. |
| ジャーナル | BMC Mol Biol |
| Abstract |
BACKGROUND:G-quadruplex is a DNA secondary structure that has been shown to play an important role in biological systems. In a previous study, we identified 1998 G-quadruplex-forming sequences using a mouse CpG islands DNA microarray with a fluorescent-labeled G-quadruplex ligand. Among these putative G-quadruplex-forming sequences, G-quadruplex formation was verified for 10 randomly selected sequences by CD spectroscopy and DMS footprinting analysis. In this study, the biological function of the 10 G-quadruplex-forming sequences in the transcriptional regulation has been analyzed using a reporter assay. RESULTS:When G-quadruplex-forming sequences from the Dele and Cdc6 genes have been cloned in reporter vectors carrying a minimal promoter and the luciferase gene, luciferase expression is activated. This has also been detected in experiments applying a promoterless reporter vector. Mutational analysis reveals that guanine bases, which form the G-tetrads, are important in the activation. In addition, the activation has been found to decrease by the telomestatin derivative L1H1-7OTD which can bind to the G-quadruplex DNA. When Dele and Cdc6 CpG islands, containing the G-quadruplex-forming sequence, have been cloned in the promoterless reporter vector, the luciferase expression is activated. Mutational analysis reveals that the expression level is decreased by mutation on Dele G-quadruplex; however, increased by mutation on Cdc6 G-quadruplex. CONCLUSION:Dele and Cdc6 G-quadruplex formation is significant in the transcriptional regulation. Dele and Cdc6 G-quadruplex DNA alone possess enhancer and promotor function. When studied in more complex CpG islands Dele G-quadruplex also demonstrates promotor activity, whereas Cdc6 G-quadruplex may possess a dual function of transcriptional regulation. |
| 巻・号 | 18(1) |
| ページ | 17 |
| 公開日 | 2017-6-27 |
| DOI | 10.1186/s12867-017-0094-z |
| PII | 10.1186/s12867-017-0094-z |
| PMID | 28655335 |
| PMC | PMC5488298 |
| MeSH | Animals Cell Cycle Proteins / genetics* Circular Dichroism CpG Islands* G-Quadruplexes* Gene Expression Gene Expression Regulation* Genes, Reporter Mice Mitochondrial Proteins / genetics* NIH 3T3 Cells Nuclear Proteins / genetics* Transcription, Genetic* |
| IF | 1.658 |
| 引用数 | 5 |
| リソース情報 | |
| ヒト・動物細胞 | NIH3T3-3-4(RCB1862) |