論文 - 詳細
| RRC ID | 52279 |
|---|---|
| 著者 | Suzuki R, Sakagami H, Amano S, Fukuchi K, Sunaga K, Kanamoto T, Terakubo S, Nakashima H, Shirataki Y, Tomomura M, Masuda Y, Yokose S, Tomomura A, Watanabe H, Okawara M, Matahira Y. |
| タイトル | Evaluation of Biological Activity of Mastic Extracts Based on Chemotherapeutic Indices. |
| ジャーナル | In Vivo |
| Abstract |
BACKGROUND:Most previous mastic investigators have not considered its potent cytotoxicity that may significantly affect the interpretation of obtained data. In the present study, we re-evaluated several biological activities of mastic extracts, based on chemotherapeutic indexes. MATERIALS AND METHODS:Pulverized mastic gum was extracted with n-hexane and then with ethyl acetate or independently with methanol or n-butanol. Tumor specificity (TS) of the extracts was determined by their cytotoxicity against human malignant and non-malignant cells. Antibacterial activity was determined by their cytotoxicity against bacteria and normal oral cells. Antiviral activity was determined by their protection of viral infection and cytotoxic activity. Cytochrome P-450 (CYP) 3A4 activity was measured by β-hydroxylation of testosterone. RESULTS:Ethyl acetate extract showed slightly higher tumor specificity (TS=2.6) and one order higher antibacterial activity (selectivity index (SI)=0.813) than other extracts (TS=1.4-2.5; SI=0.030-0.063). All extracts showed no anti-human immunodeficiency virus (HIV) activity, but some anti-herpes simplex virus (HSV) activity, which was masked by potent cytotoxicity. They showed strong inhibitory activity against CYP3A4. CONCLUSION:Ethyl acetate extraction following the removal of cytotoxic and CYP3A4 inhibitory substances by n-hexane can enhance antitumor and antibacterial activity of mastic. |
| 巻・号 | 31(4) |
| ページ | 591-598 |
| 公開日 | 2017-1-1 |
| DOI | 10.21873/invivo.11099 |
| PII | 31/4/591 |
| PMID | 28652425 |
| PMC | PMC5566908 |
| MeSH | Anti-Bacterial Agents / chemistry Anti-Bacterial Agents / pharmacology Antiviral Agents / chemistry Antiviral Agents / pharmacology Bacteria / drug effects* Bacteria / pathogenicity Cell Proliferation / drug effects Cytochrome P-450 CYP3A / genetics HIV / drug effects HIV / pathogenicity Hexanes / chemistry Humans Mastic Resin / chemistry Mastic Resin / pharmacology* Neoplasms / drug therapy* Neoplasms / pathology Pistacia / chemistry Plant Extracts / chemistry Plant Extracts / pharmacology* Simplexvirus / drug effects Simplexvirus / pathogenicity |
| IF | 1.541 |
| 引用数 | 5 |
| リソース情報 | |
| ヒト・動物細胞 | HSC-2(RCB1945) HSC-3(RCB1975) HSC-4(RCB1902) |