論文 - 詳細
| RRC ID | 52289 |
|---|---|
| 著者 | Ouji Y, Sakagami M, Omori H, Higashiyama S, Kawai N, Kitahara T, Wanaka A, Yoshikawa M. |
| タイトル | Efficient induction of inner ear hair cell-like cells from mouse ES cells using combination of Math1 transfection and conditioned medium from ST2 stromal cells. |
| ジャーナル | Stem Cell Res |
| Abstract |
We sought to establish a more efficient technique for induction of inner ear hair cell-like cells (HC-like cells) from embryonic stem cells (ES cells) by using a combination of two previously reported methods; ST2 stromal cell-conditioned medium, known to be favorable for HC-like cell induction (HIST2 method), and ES cells with transfer of the Math1 gene (Math1-ES cells). Math1-ES cells carrying Tet-inducible Math1 were cultured for 14days with doxycycline in conditioned medium from cultures of ST2 stromal cells following formation of 4-day embryoid bodies (EBs). Although each of the previously introduced methods have been reported to induce approximately 20% HC-like cells and 10% HC-like cells in their respective populations in EB outgrowths at the end of the culture periods, the present combined method was able to generate approximately 30% HC-like cells expressing HC-related markers (myosin6, myosin7a, calretinin, α9AchR, Brn3c), which showed remarkable formation of stereocilia-like structures. Analysis of expressions of marker genes specific for cochlear (Lmod3, Emcn) and vestibular (Dnah5, Ptgds) cells indicated that our HIST2 method may lead to induction of cochlear- and vestibular-type cells. In addition, continuous Math1 induction by doxycycline without use of the HIST2 method preferentially induced cochlear markers with negligible effects on vestibular marker induction. |
| 巻・号 | 23 |
| ページ | 50-56 |
| 公開日 | 2017-8-1 |
| DOI | 10.1016/j.scr.2017.06.013 |
| PII | S1873-5061(17)30121-6 |
| PMID | 28689068 |
| MeSH | Animals Basic Helix-Loop-Helix Transcription Factors / genetics* Basic Helix-Loop-Helix Transcription Factors / metabolism Biomarkers / metabolism Cell Line Cells, Cultured Cochlea / cytology Culture Media, Conditioned / pharmacology* Embryoid Bodies / cytology Embryoid Bodies / drug effects Gene Expression Regulation / drug effects Hair Cells, Auditory, Inner / cytology* Mechanotransduction, Cellular Mice Mouse Embryonic Stem Cells / cytology* Myosins / metabolism Stereocilia / metabolism Stromal Cells / metabolism Transfection* Vestibule, Labyrinth / cytology |
| IF | 4.495 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |