Reference - Detail
|Author||Sangkanjanavanich N, Kawai M, Kakuda T, Takai S.|
|Title||Rescue of an intracellular avirulent Rhodococcus equi replication defect by the extracellular addition of virulence-associated protein A.|
|Journal||J Vet Med Sci|
Rhodococcus equi is a facultative intracellular bacterium that can escape from bactericidal mechanisms associated with phagocytosis. Virulence-associated protein A (VapA), encoded on a virulence-associated plasmid, is essential for intracellular survival in macrophages, but its function is not known. Here, we show that the extracellular addition of recombinant glutathione S-transferase (GST)-VapA fusion protein rescued the intracellular replication defect of a mutant lacking the vapA gene. Furthermore, the virulence-plasmid-cured strain could also multiply to nearly wild-type levels by the addition of GST-VapA. The present data suggest that VapA can alter the intraphagocytic environment, thereby affecting its suitability for the growth of R. equi.
|MeSH||Actinomycetales Infections / microbiology Animals Cell Line Gene Deletion Genes, Bacterial / genetics Glutathione Transferase Macrophages / microbiology Mice Plasmids Recombinant Proteins Rhodococcus equi / genetics* Rhodococcus equi / growth & development Rhodococcus equi / pathogenicity* Staphylococcal Protein A / genetics* Virulence / genetics* Virulence Factors*|
|Human and Animal Cells||J774.1(RCB0434)|