RRC ID 52306
Author Sangkanjanavanich N, Kawai M, Kakuda T, Takai S.
Title Rescue of an intracellular avirulent Rhodococcus equi replication defect by the extracellular addition of virulence-associated protein A.
Journal J Vet Med Sci
Abstract Rhodococcus equi is a facultative intracellular bacterium that can escape from bactericidal mechanisms associated with phagocytosis. Virulence-associated protein A (VapA), encoded on a virulence-associated plasmid, is essential for intracellular survival in macrophages, but its function is not known. Here, we show that the extracellular addition of recombinant glutathione S-transferase (GST)-VapA fusion protein rescued the intracellular replication defect of a mutant lacking the vapA gene. Furthermore, the virulence-plasmid-cured strain could also multiply to nearly wild-type levels by the addition of GST-VapA. The present data suggest that VapA can alter the intraphagocytic environment, thereby affecting its suitability for the growth of R. equi.
Volume 79(8)
Pages 1323-1326
Published 2017-8-4
DOI 10.1292/jvms.17-0350
PMID 28690290
PMC PMC5573816
MeSH Actinomycetales Infections / microbiology Animals Cell Line Gene Deletion Genes, Bacterial / genetics Glutathione Transferase Macrophages / microbiology Mice Plasmids Recombinant Proteins Rhodococcus equi / genetics* Rhodococcus equi / growth & development Rhodococcus equi / pathogenicity* Staphylococcal Protein A / genetics* Virulence / genetics* Virulence Factors*
IF 0.803
Times Cited 2
Human and Animal Cells J774.1(RCB0434)