Reference - Detail
| RRC ID | 52314 |
|---|---|
| Author | Wang P, Ying J, Luo C, Jin X, Zhang S, Xu T, Zhang L, Mi M, Chen D, Tong P, Jin H. |
| Title | Osthole Promotes Bone Fracture Healing through Activation of BMP Signaling in Chondrocytes. |
| Journal | Int J Biol Sci |
| Abstract |
Osthole is a bioactive coumarin derivative and has been reported to be able to enhance bone formation and improve fracture healing. However, the molecular mechanism of Osthole in bone fracture healing has not been fully defined. In this study we determined if Osthole enhances bone fracture healing through activation of BMP2 signaling in mice. We performed unilateral open transverse tibial fracture procedure in 10-week-old C57BL/6 mice which were treated with or without Osthole. Our previous studies demonstrated that chondrocyte BMP signaling is required for bone fracture healing, in this study we also performed tibial fracture procedure in Cre-negative and Col2-Cre;Bmp2flox/flox conditional knockout (KO) mice (Bmp2Col2Cre ) to determine if Osthole enhances fracture healing in a BMP2-dependent manner. Fracture callus tissues were collected and analyzed by X-ray, micro-CT (μCT), histology, histomorphometry, immunohistochemistry (IHC), biomechanical testing and quantitative gene expression analysis. In addition, mouse chondrogenic ATDC5 cells were cultured with or without Osthole and the expression levels of chondrogenic marker genes were examined. The results demonstrated that Osthole promotes bone fracture healing in wild-type (WT) or Cre- control mice. In contrast, Osthole failed to promote bone fracture healing in Bmp2Col2Cre conditional KO mice. In the mice receiving Osthole treatment, expression of cartilage marker genes was significantly increased. We conclude that Osthole could promote bone strength and enhance fracture healing by activation of BMP2 signaling. Osthole may be used as an alternative approach in the orthopaedic clinic for the treatment of fracture healing. |
| Volume | 13(8) |
| Pages | 996-1007 |
| Published | 2017-7-18 |
| DOI | 10.7150/ijbs.19986 |
| PII | ijbsv13p0996 |
| PMID | 28924381 |
| PMC | PMC5599905 |
| MeSH | Animals Bone Morphogenetic Proteins / metabolism* Chondrocytes / cytology Chondrocytes / drug effects Coumarins / therapeutic use* Fracture Healing / drug effects Gene Expression Regulation / drug effects Male Mice Mice, Inbred C57BL Osteogenesis / drug effects Signal Transduction / drug effects Tibial Fractures / drug therapy* |
| IF | 4.858 |
| Times Cited | 12 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Youtube |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | ATDC5(RCB0565) |