RRC ID 52317
Author Rodriguez R, Yoshimura K, Shibata Y, Miyamoto Y, Tanaka R, Uyama R, Sasa K, Suzuki D, Miyazaki T, Kamijo R.
Title Nanoindentation time-dependent deformation/recovery suggestive of methylglyoxal induced glycation in calcified nodules.
Journal Nanomedicine
Abstract Although empirical findings have indicated increase in bone fracture risk in type 2 diabetes patients, that has yet to be proven by results obtained at the material level. Here, we report evidence showing nanoscale time-dependent deformation/recovery of in vitro calcified nodules mimicking bone turnover in type 2 diabetes in respect to methylglyoxal (MG)-induced glycation. Nanoindentation test results revealed that calcified nodules cultured with MG did not show adequate dimensional recovery, despite a large creep rate during constant load indentation testing. This lesser recovery is likely based on the linear matrix polymerization network formed by advanced glycation end products (AGEs) as a secondary product of MG. Since elevated serum MG and abnormal bone turnover related to the amount of AGEs are observed in cases of type 2 diabetes, this time-dependent behavior may be one of the factors of the bone fracture mechanism at the material level in affected patients.
Volume 13(8)
Pages 2545-2553
Published 2017-11
DOI 10.1016/j.nano.2017.07.003
PII S1549-9634(17)30133-8
PMID 28754466
MeSH Bone and Bones / metabolism Bone and Bones / pathology Calcinosis / metabolism* Calcinosis / pathology Cell Line Cell Proliferation Diabetes Mellitus, Type 2 / metabolism* Diabetes Mellitus, Type 2 / pathology Glycation End Products, Advanced / metabolism* Humans Osteoblasts / cytology Osteoblasts / metabolism* Osteoblasts / pathology Pyruvaldehyde / metabolism*
IF 5.57
Resource
Human and Animal Cells MC3T3-E1(RCB1126)